Anti-ACE2 antibody (ab15348)
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Specificity
No cross reactivity to ACE1.
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Tested applications
Suitable for: IHC-Fr, ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat -
Immunogen
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Positive control
- WB: Human kidney, testis, lung, intestine and breast tissue lysates; Caco-2 cell lysate; Mouse liver, spleen, heart, bladder, pancreas and stomach tissue lysates; Rat skin, spleen, heart and brain tissue lysates. IHC-P: Fruit bat nasal turbinate, trachea, lung, kidney, intestine and stomach tissues; Human thoracic aortic tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
No cross reactivity to ACE1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab15348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Angiotensin Converting Enzyme 2 peptide (ab15352). | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Human testis tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human intestine tissue lysate
Lane 4 : Human breast tissue lysate
Lane 5 : Caco2 cell lysateLysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa -
Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)4% paraformaldehyde-fixed human lung tissue stained for ACE2 with ab15348 at a 20 μg/mL dilution in immunofluorescence analysis.
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Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)4% paraformaldehyde-fixed human testis tissue stained for ACE2 with ab15348 at a 20 μg/mL dilution in immunofluorescence analysis.
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Rat skin tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDaWestern blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse bladder tissue lysate
Lane 5 : Mouse pancreas tissue lysate
Lane 6 : Mouse stomach tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDaWestern blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
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Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)4% paraformaldehyde-fixed mouse lung tissue stained for ACE2 with ab15348 at a 20 μg/mL dilution in immunofluorescence analysis.
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Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)4% paraformaldehyde-fixed rat lung tissue stained for ACE2 with ab15348 at a 20 μg/mL dilution in immunofluorescence analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(A).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat nasal turbinate—400×, multifocal apical immunoreactivity in ciliated epithelial cells (arrows).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(B).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat trachea—400×, negative immunostaining.
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(C).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat lung—400×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(D).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat kidney—200×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(E).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat stomach—400×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(F).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat intestine—100×, diffuse immunoreactivity of brush border.
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)This image is courtesy of an anonymous Abreviewab15348 staining ACE2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)ab15348 at 2µg/ml staining ACE2 in human kidney by immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protocols
References
ab15348 has been referenced in 18 publications.
- Gandhi CK et al. Degradation of Lung Protective Angiotensin Converting Enzyme-2 by Meconium in Human Alveolar Epithelial Cells: A Potential Pathogenic Mechanism in Meconium Aspiration Syndrome. Lung 197:227-233 (2019). PubMed: 30759273
- Zhang L et al. Propofol prevents human umbilical vein endothelial cell injury from Ang II-induced apoptosis by activating the ACE2-(1-7)-Mas axis and eNOS phosphorylation. PLoS One 13:e0199373 (2018). PubMed: 29995907
- van Doremalen N et al. SARS-Like Coronavirus WIV1-CoV Does Not Replicate in Egyptian Fruit Bats (Rousettus aegyptiacus). Viruses 10:N/A (2018). IHC-P ; Bat . PubMed: 30572566
- Delforce SJ et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). PubMed: 27956412
- Wang J et al. The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction. Mol Med Rep 16:1973-1981 (2017). PubMed: 28656296
