Anti-ACE2 antibody (ab15348)
Key features and details
- Rabbit polyclonal to ACE2
- Suitable for: IHC-Fr, IHC (PFA fixed), WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Specificity
No cross reactivity to ACE1.
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Tested applications
Suitable for: IHC-Fr, IHC (PFA fixed), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BYF1
(Peptide available asab15352) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
No cross reactivity to ACE1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| IHC (PFA fixed) |
Use at an assay dependent concentration.
|
|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human ACE2 peptide (ab15352).
|
| IHC-P | (5) |
Use a concentration of 2 µg/ml.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC (PFA fixed)
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human ACE2 peptide (ab15352). |
|
IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Western blot - Anti-ACE2 antibody (ab15348)
All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Human testis tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human intestine tissue lysate
Lane 4 : Human breast tissue lysate
Lane 5 : Caco2 cell lysateLysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Rat skin tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDaWestern blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse bladder tissue lysate
Lane 5 : Mouse pancreas tissue lysate
Lane 6 : Mouse stomach tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDaWestern blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (PFA fixed) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.
Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab15348 has been referenced in 26 publications.
- Collin J et al. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf 19:190-200 (2021). PubMed: 32502616
- Yamamoto M et al. The Anticoagulant Nafamostat Potently Inhibits SARS-CoV-2 S Protein-Mediated Fusion in a Cell Fusion Assay System and Viral Infection In Vitro in a Cell-Type-Dependent Manner. Viruses 12:N/A (2020). PubMed: 32532094
- Wang Q et al. E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR a7 receptor: role of nAChR a7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation. Respir Res 21:154 (2020). PubMed: 32552811
- Nicin L et al. Cell type-specific expression of the putative SARS-CoV-2 receptor ACE2 in human hearts. Eur Heart J 41:1804-1806 (2020). PubMed: 32293672
- Leung JM et al. ACE-2 expression in the small airway epithelia of smokers and COPD patients: implications for COVID-19. Eur Respir J 55:N/A (2020). PubMed: 32269089