Anti-ACE2 antibody (ab15348)
- Datasheet
- References (18)
- Protocols
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Cat, Human, Ferret, Macaque monkey, Bat -
Immunogen
Synthetic peptide corresponding to Human ACE2 aa 788-805 (C terminal).
Sequence:CKGENNPGFQNTDDVQTSF
(Peptide available asab15352) -
Positive control
- WB: Human kidney and testis cell lysates.
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General notes
No cross reactivity to ACE1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
No cross reactivity to ACE1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab15348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- Unigene: 178098 Human
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Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(A).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat nasal turbinate—400×, multifocal apical immunoreactivity in ciliated epithelial cells (arrows).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(B).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat trachea—400×, negative immunostaining.
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(C).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat lung—400×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(D).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat kidney—200×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(E).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat stomach—400×, multifocal cytoplasmic endothelial cell immunoreactivity (arrowheads).
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Image from van Doremalen N et al., Viruses. 2018;10(12):E727. Fig 2(F).; 10.3390/v10120727.ACE2 immunohistochemistry of fruit bat intestine—100×, diffuse immunoreactivity of brush border.
Tissues were fixed in 10% neutral buffered formalin for a minimum of seven days. Sagittal-cut skulls were decalcified in 20% EDTA in sucrose, changed weekly for six weeks. Tissues were then processed using a Sakura VIP-6 Tissue Tek tissue processor and embedded in Ultraffin paraffin polymer. Samples were sectioned at 5µm, and resulting slides were stained with hematoxylin and eosin.
Tissues were stained with ab15348, at a 1:1500 dilution. Antibodies were detected using ImmPress VR Polymer HRP anti-rabbit IgG followed by Discovery ChromoMap DAB kit.
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Immunohistochemistry (Frozen sections) - Anti-ACE2 antibody (ab15348)This image is courtesy of an anonymous Abreviewab15348 staining ACE2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody (ab15348)ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-ACE2 antibody (ab15348)Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)ab15348 at 2µg/ml staining ACE2 in human kidney by immunohistochemistry.
Protocols
References
This product has been referenced in:
- Gandhi CK et al. Degradation of Lung Protective Angiotensin Converting Enzyme-2 by Meconium in Human Alveolar Epithelial Cells: A Potential Pathogenic Mechanism in Meconium Aspiration Syndrome. Lung 197:227-233 (2019). Read more (PubMed: 30759273) »
- Zhang L et al. Propofol prevents human umbilical vein endothelial cell injury from Ang II-induced apoptosis by activating the ACE2-(1-7)-Mas axis and eNOS phosphorylation. PLoS One 13:e0199373 (2018). Read more (PubMed: 29995907) »
