Anti-ACE2 antibody (ab15348)
Key features and details
- Rabbit polyclonal to ACE2
- Suitable for: ICC/IF, ELISA, IHC (PFA fixed), IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Specificity
No cross reactivity to ACE1.
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Tested applications
Suitable for: ICC/IF, ELISA, IHC (PFA fixed), IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat -
Immunogen
Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BYF1 -
Positive control
- WB: Human testis, lung, intestine, breast and kidney tissue lysates. Caco2 cell lysate. Rat skin, spleen, heart and brain tissue lysates. Mouse liver, spleen, heart, bladder, pancreas and stomach tissue lysates. IHC-P: Human lung, testis and kidney tissue. Rat lung tissue. Mouse lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
No cross reactivity to ACE1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC (PFA fixed) |
Use at an assay dependent concentration.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| WB | (5) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).
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| IHC-P | (5) |
Use a concentration of 2 µg/ml.
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| Notes |
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ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC (PFA fixed)
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). |
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IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 1/1000 dilution
Lane 1 : Wild-type Caco-2 cell lysate
Lane 2 : ACE2 knockout Caco-2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120-135 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ACE2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15348 was shown to bind specifically to ACE2. A band was observed at 120-135 kDa in wild-type Caco-2 cell lysates with no signal observed at this size in ACE2 knockout cell line ab277338 (knockout cell lysate ab275516). To generate this image, wild-type and ACE2 knockout Caco-2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Human Testis Lysate
Lane 2 : Human Lung lysate
Lane 3 : Human intestine lysate
Lane 4 : Human Breast lysate
Lane 5 : Caco2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Rat skin tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse bladder tissue lysate
Lane 5 : Mouse pancreas tissue lysate
Lane 6 : Mouse stomach tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
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Western blot - Anti-ACE2 antibody (ab15348)Lane 1 : Anti-ACE2 antibody (ab15348) at 0.5 µg/ml
Lane 2 : Anti-ACE2 antibody (ab15348) at 1 µg/ml
Lane 3 : Anti-ACE2 antibody (ab15348) at 2 µg/ml
All lanes : Human Kidney Lysate
Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.
Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (109)
ab15348 has been referenced in 109 publications.
- Collin J et al. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf 19:190-200 (2021). PubMed: 32502616
- Patankar JV et al. Severe Acute Respiratory Syndrome Coronavirus 2 Attachment Receptor Angiotensin-Converting Enzyme 2 Is Decreased in Crohn's Disease and Regulated By Microbial and Inflammatory Signaling. Gastroenterology 160:925-928.e4 (2021). PubMed: 33075345
- Eapen MS et al. Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections. Am J Physiol Lung Cell Mol Physiol 320:L158-L163 (2021). PubMed: 33174446
- Chadchan SB et al. The SARS-CoV-2 receptor, angiotensin-converting enzyme 2, is required for human endometrial stromal cell decidualization†. Biol Reprod 104:336-343 (2021). PubMed: 33205194
- Kajiwara K et al. Immunohistochemical study for the expression of leukocyte adhesion molecules, and FGF23 and ACE2 in P. gingivalis LPS-induced diabetic nephropathy. BMC Nephrol 22:3 (2021). PubMed: 33407253