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    ace2-antibody-ab15348.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Metalloprotease ACEs
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Anti-ACE2 antibody (ab15348)

  • Datasheet
  • SDS
Reviews (10)Q&A (2)References (26)

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Western blot - Anti-ACE2 antibody (ab15348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
  • Western blot - Anti-ACE2 antibody (ab15348)
  • Western blot - Anti-ACE2 antibody (ab15348)
  • Western blot - Anti-ACE2 antibody (ab15348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
  • Immunohistochemistry (PFA fixed) - Anti-ACE2 antibody (ab15348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)

Key features and details

  • Rabbit polyclonal to ACE2
  • Suitable for: IHC-Fr, IHC (PFA fixed), WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ACE2 antibody
    See all ACE2 primary antibodies
  • Description

    Rabbit polyclonal to ACE2
  • Host species

    Rabbit
  • Specificity

    No cross reactivity to ACE1.

  • Tested applications

    Suitable for: IHC-Fr, IHC (PFA fixed), WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9BYF1
    (Peptide available as ab15352)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    No cross reactivity to ACE1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ACEs
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Signal Transduction
    • Antibodies
    • ace2

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human ACE2 peptide (ab15352)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ACE2 protein (ab151852)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
IHC (PFA fixed)
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human ACE2 peptide (ab15352).
IHC-P (5)
Use a concentration of 2 µg/ml.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC (PFA fixed)
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human ACE2 peptide (ab15352).
IHC-P
Use a concentration of 2 µg/ml.

Target

  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59272 Human
    • Entrez Gene: 70008 Mouse
    • Entrez Gene: 302668 Rat
    • Omim: 300335 Human
    • SwissProt: Q9BYF1 Human
    • SwissProt: Q8R0I0 Mouse
    • SwissProt: Q5EGZ1 Rat
    • Unigene: 178098 Human
    • Unigene: 13451 Mouse
    • Unigene: 129779 Rat
    see all
  • Alternative names

    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Images

  • Western blot - Anti-ACE2 antibody (ab15348)
    Western blot - Anti-ACE2 antibody (ab15348)

    All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Human intestine tissue lysate
    Lane 4 : Human breast tissue lysate
    Lane 5 : Caco2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

    Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.

    Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

  • Western blot - Anti-ACE2 antibody (ab15348)
    Western blot - Anti-ACE2 antibody (ab15348)
    All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml

    Lane 1 : Rat skin tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 97 kDa



    Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.

  • Western blot - Anti-ACE2 antibody (ab15348)
    Western blot - Anti-ACE2 antibody (ab15348)
    All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse bladder tissue lysate
    Lane 5 : Mouse pancreas tissue lysate
    Lane 6 : Mouse stomach tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 97 kDa



    Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.

  • Western blot - Anti-ACE2 antibody (ab15348)
    Western blot - Anti-ACE2 antibody (ab15348)

    Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5  μg/mL (lane A), 1  μg/mL (lane B), and 2  μg/mL (lane C).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

    Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

  • Immunohistochemistry (PFA fixed) - Anti-ACE2 antibody (ab15348)
    Immunohistochemistry (PFA fixed) - Anti-ACE2 antibody (ab15348)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.

    Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.

    Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (26)

Publishing research using ab15348? Please let us know so that we can cite the reference in this datasheet.

ab15348 has been referenced in 26 publications.

  • Collin J  et al. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf 19:190-200 (2021). PubMed: 32502616
  • Yamamoto M  et al. The Anticoagulant Nafamostat Potently Inhibits SARS-CoV-2 S Protein-Mediated Fusion in a Cell Fusion Assay System and Viral Infection In Vitro in a Cell-Type-Dependent Manner. Viruses 12:N/A (2020). PubMed: 32532094
  • Wang Q  et al. E-cigarette-induced pulmonary inflammation and dysregulated repair are mediated by nAChR a7 receptor: role of nAChR a7 in SARS-CoV-2 Covid-19 ACE2 receptor regulation. Respir Res 21:154 (2020). PubMed: 32552811
  • Nicin L  et al. Cell type-specific expression of the putative SARS-CoV-2 receptor ACE2 in human hearts. Eur Heart J 41:1804-1806 (2020). PubMed: 32293672
  • Leung JM  et al. ACE-2 expression in the small airway epithelia of smokers and COPD patients: implications for COVID-19. Eur Respir J 55:N/A (2020). PubMed: 32269089
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization
Yes - 0.05% tween 20 in the washing buffer
Specification
Mouse kidney
Blocking step
commercial - Invitrogen's CAS-Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Adult Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization
Yes - 0.05% tween 20 in the washing buffer
Specification
Adult Kidney
Blocking step
commercial - Invitrogen's CAS-Block as blocking as blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Ferret Tissue sections (respiratory tract)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Specification
respiratory tract
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 08 2008

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (respiratory tract)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Specification
respiratory tract
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 08 2008

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensin Converting Enzyme 2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%
Read More

Miss. Laura Palmer

Verified customer

Submitted May 22 2007

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