Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization
Yes - 0.05% tween 20 in the washing buffer
Specification
Mouse kidney
Blocking step
commercial - Invitrogen's CAS-Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: commercial - Thermo's antibody diluent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Vector MP-7401: ImmPRESS™ HRP Anti-Rabbit IgG
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted May 13 2020