Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in PBST
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview for this product. Recent retests of ab15348 in immunocytochemistry/immunofluorescence (ICC/IF) showed cytoplasmic staining, instead of expected staining of the membrane. Therefore, we concluded that this product is not suitable for ICC/IF and have now removed this application from the datasheet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
Submitted Aug 03 2020