Application
Western blot
Sample
Human Cell lysate - whole cell (Dermal microvascular endothelial cells)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
20 µg
Treatment
TNF-a 20 ng/mL for 24 h
Specification
Dermal microvascular endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55, 95, 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Rami Najjar
Verified customer
Submitted Sep 28 2022