Anti-ACE2 antibody (ab87436)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cat, Dog, Orangutan
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Immunogen
Synthetic peptide corresponding to Human ACE2 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87437) -
Positive control
- This antibody gave a positive signal in the following tissue lysate: Human Kidney This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ACE2 antibody (ab87436)ICC/IF image of ab87436 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87436 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-ACE2 antibody (ab87436)Anti-ACE2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 115 kDa (possible glycosylated form)
Exposure time: 30 secondsACE2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Joshi S et al. Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34+ cells. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30989646) »
- Min JJ et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). Read more (PubMed: 29682576) »
