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    ace2-antibody-ab87436.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Metalloprotease ACEs
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Anti-ACE2 antibody (ab87436)

  • Datasheet
Reviews (4) Submit a question References (7)

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Abpromise

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Western blot - Anti-ACE2 antibody (ab87436)
  • Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436)

Key features and details

  • Rabbit polyclonal to ACE2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ACE2 antibody
    See all ACE2 primary antibodies
  • Description

    Rabbit polyclonal to ACE2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human ACE2 aa 350-450 conjugated to keyhole limpet haemocyanin.
    Database link: Q9BYF1
    (Peptide available as ab87437)

  • Positive control

    • WB: Human kidney tissue lysate (ab30203). IHC-P: Mouse heart tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ACEs
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ACE2 protein (ab151852)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab87436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Hamster
Cat
Dog
Orangutan
Application Abreviews Notes
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 59272 Human
      • Entrez Gene: 70008 Mouse
      • Entrez Gene: 302668 Rat
      • Omim: 300335 Human
      • SwissProt: Q9BYF1 Human
      • SwissProt: Q8R0I0 Mouse
      • SwissProt: Q5EGZ1 Rat
      • Unigene: 178098 Human
      • Unigene: 13451 Mouse
      • Unigene: 129779 Rat
      see all
    • Alternative names

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    Images

    • Western blot - Anti-ACE2 antibody (ab87436)
      Western blot - Anti-ACE2 antibody (ab87436)
      Anti-ACE2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 92 kDa
      Additional bands at: 115 kDa (possible glycosylated form)


      Exposure time: 30 seconds


      ACE2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.

    • Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436)
      Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436)This image is courtesy of an anonymous Abreview.

      ab87436 staining ACE2 in mouse heart tissue sections by immunohistochemistry. Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with ab87436 (1/100 dilution) for 18 hours at 4oC. A HRP-conjugated goat anti-rabbit polyclonal (1/50) was used as the secondary antibody.

      See Abreview

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab87436? Please let us know so that we can cite the reference in this datasheet.

    ab87436 has been referenced in 7 publications.

    • Joshi S  et al. Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34+ cells. J Cell Physiol N/A:N/A (2019). PubMed: 30989646
    • Min JJ  et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). PubMed: 29682576
    • Lee J  et al. Enteroendocrine-derived glucagon-like peptide-2 controls intestinal amino acid transport. Mol Metab 6:245-255 (2017). PubMed: 28271031
    • Gao H  et al. A Low-Protein Diet Enhances Angiotensin II Production in the Lung of Pregnant Rats but not Nonpregnant Rats. J Pregnancy 2016:4293431 (2016). WB . PubMed: 27195150
    • Gao H  et al. Gestational protein restriction increases angiotensin II production in rat lung. Biol Reprod 88:64 (2013). PubMed: 23365412
    • Gao H  et al. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod 86:31 (2012). WB, IHC-P ; Rat . PubMed: 22011389
    • Gao H  et al. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod 86:68 (2012). WB ; Rat . PubMed: 22088913

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-ACE2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    90 µg
    Specification
    lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. xiaowen cheng

    Verified customer

    Submitted Feb 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

    Western blot abreview for Anti-ACE2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    25 µg
    Specification
    cardiomyocytes
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Prof. Paul Townsend

    Verified customer

    Submitted Mar 24 2010

    Western blot abreview for Anti-ACE2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestine, Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Intestine, Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Shunyou Wang

    Verified customer

    Submitted Mar 03 2010

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