Anti-ACE2 antibody (ab87436)
Key features and details
- Rabbit polyclonal to ACE2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACE2 antibody
See all ACE2 primary antibodies -
Description
Rabbit polyclonal to ACE2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- WB: Human kidney tissue lysate (ab30203). IHC-P: Mouse heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| All applications |
Mouse
Rat
Hamster
Cat
Dog
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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Western blot - Anti-ACE2 antibody (ab87436)Anti-ACE2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 115 kDa (possible glycosylated form)
Exposure time: 30 secondsACE2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
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Immunohistochemistry paraffin embedded sections - Anti-ACE2 antibody (ab87436)This image is courtesy of an anonymous Abreview.ab87436 staining ACE2 in mouse heart tissue sections by immunohistochemistry. Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 1 hour at 25oC; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with ab87436 (1/100 dilution) for 18 hours at 4oC. A HRP-conjugated goat anti-rabbit polyclonal (1/50) was used as the secondary antibody.
Protocols
Datasheets and documents
References (7)
ab87436 has been referenced in 7 publications.
- Joshi S et al. Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34+ cells. J Cell Physiol N/A:N/A (2019). PubMed: 30989646
- Min JJ et al. Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control. J Diabetes Res 2018:1067853 (2018). PubMed: 29682576
- Lee J et al. Enteroendocrine-derived glucagon-like peptide-2 controls intestinal amino acid transport. Mol Metab 6:245-255 (2017). PubMed: 28271031
- Gao H et al. A Low-Protein Diet Enhances Angiotensin II Production in the Lung of Pregnant Rats but not Nonpregnant Rats. J Pregnancy 2016:4293431 (2016). WB . PubMed: 27195150
- Gao H et al. Gestational protein restriction increases angiotensin II production in rat lung. Biol Reprod 88:64 (2013). PubMed: 23365412
- Gao H et al. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod 86:31 (2012). WB, IHC-P ; Rat . PubMed: 22011389
- Gao H et al. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod 86:68 (2012). WB ; Rat . PubMed: 22088913
