Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ACE2 antibody [EPR24705-45] (ab272500)

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Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunoprecipitation - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
  • Anti-ACE2 antibody [EPR24705-45] (ab272500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24705-45] to ACE2
  • Suitable for: Flow Cyt, IP, WB, ICC, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-ACE2 antibody [EPR24705-45]
    See all ACE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24705-45] to ACE2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and testis tissue lysates; Calu-3 whole cell lysate. IHC-P: Human kidney and testis tissues. ICC: Calu-3, HEK-293T cells transfected with myc-ACE-2; WT CaCo2. Flow cyt: Calu-3 cells. IP: Human testis tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
1/500.
IP
1/30.
WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
ICC
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt
1/500.
IP
1/30.
WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
ICC
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

  • Sequence similarities

    Belongs to the peptidase M2 family.

  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    Images

    • Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      ab272500 staining ACE2 in wild-type Caco2 cells (top panel) and ACE2 knockout Caco2 cells (ab273731) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab272500 at 10μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Membranous and cytoplasmic staining in human testis. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling ACE2 with ab272500 at 1/250 (2.0 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with myc-tagged ACE2 expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunocytochemistry - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Calu-3 cells labelling ACE2 with ab272500 at 1/50 (10.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of Calu-3 cells. Negative control: HeLa (PMID: 32926098). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Red) was used at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Flow Cytometry - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left)/ Calu-3 (human lung adenocarcinoma epithelial cell, Right) cells labelling ACE2 with ab272500 at 1/500 dilution (0.1ug). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

      Negative control: HeLa (PMID: 32926098). Gated on viable cells.

    • Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      All lanes : Anti-ACE2 antibody [EPR24705-45] (ab272500) at 1/1000 dilution

      Lane 1 : Calu-3 (Human lung adenocarcinoma epithelial cell) whole cell lysate 20
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 92 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:33008593).

      Negative control: HeLa (PMID:32926098).

      Exposure time: 3 minutes

       

    • Immunoprecipitation - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunoprecipitation - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      ACE2 was immunoprecipitated from 0.35 mg Human testis lysate with ab272500 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272500 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human testis lysate 10 ug

      Lane 2: ab272500 IP in Human testis lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272500 in human testis whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 seconds

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous and cytoplasmic staining in human kidney. The section was incubated with ab272500 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Western blot - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      All lanes : Anti-ACE2 antibody [EPR24705-45] (ab272500) at 1/1000 dilution

      Lane 1 : Human kidney tissue lysate
      Lane 2 : Human testis tissue lysate
      Lane 3 : Human liver tissue lysate

      Secondary
      All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

      Predicted band size: 92 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight  observed is consistent with what has been described in the literature (PMID:10924499, PMID: 32661139).

      Negative control: liver (PMID:10924499).

      Exposure time: 3 minutes

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR24705-45] (ab272500)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ACE2 with ab272500 at 1/100 (5.0 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human liver. The section was incubated with ab272500 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection) .

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-ACE2 antibody [EPR24705-45] (ab272500)
      Anti-ACE2 antibody [EPR24705-45] (ab272500)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab272500? Please let us know so that we can cite the reference in this datasheet.

    ab272500 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Flow Cytometry abreview for Anti-ACE2 antibody [EPR24705-45]

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (CaLu-3)
    Permeabilization
    No
    Gating Strategy
    Live cells
    Specification
    CaLu-3
    Preparation
    Cell harvesting/tissue preparation method: Cells were trypsinised
    Sample buffer: PBS + 5% FCS
    Read More

    Dr. Thomas Crozier

    Verified customer

    Submitted Jun 16 2021

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