Recombinant
RabMAb

Recombinant Anti-ACE2 antibody [EPR4435(2)] (ab108252)

Submit a review Q&A (6)References (14)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4435(2)] to ACE2
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ACE2 antibody [EPR4435(2)]
    See all ACE2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4435(2)] to ACE2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ACE2 aa 200-300 (extracellular). The exact sequence is proprietary.
    (Peptide available as ab198988)

  • Positive control

    • WB: Human testis, kidney and lung tissue lysates; Human fetal kidney tissue lysate; HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and testis tissues. IP: Human testis tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with ACE2 peptide (ab198988).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

    • Sequence similarities

      Belongs to the peptidase M2 family.

    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    Images

    • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

      Lane 1 : Human testis cell lysate
      Lane 2 : Human kidney cell lysate
      Lane 3 : Human lung cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Caco-2 cell lysate
      Lane 6 : A549 cell lysate (negative control)

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?



      Lanes 1 - 6: Merged signal (red and green). Green - ab108252 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.

      ab108252 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and Mouse anti Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

      ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded Human kidney tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 92 kDa

    • Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

      ab108252 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 0.6 μg primary antibody (1/20). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.

      Exposure time: 1 second.
      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.



      All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/500 dilution

      Lane 1 : Human testis tissue lysate at 10 µg
      Lane 2 : ab108252 + Human testis tissue lysate
      Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108252 in Human testis tissue lysate

      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

      ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded Human testis tissue by immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    Publishing research using ab108252? Please let us know so that we can cite the reference in this datasheet.

    ab108252 has been referenced in 14 publications.

    • Poletto Bonetto JH  et al. Impact of early life AT1 blockade on adult cardiac morpho-functional changes and the renin-angiotensin system in a model of neonatal high oxygen-induced cardiomyopathy. Eur J Pharmacol 860:172585 (2019). PubMed: 31376367
    • Lira DGD  et al. Superimposing a high-fat diet on Schistosoma mansoni infection affects renin-angiotensin system components in the mouse kidney. Rev Soc Bras Med Trop 52:e20180371 (2019). PubMed: 30843967
    • Ichikawa H  et al. Blood Pressure-Independent Effect of Olmesartan on Albuminuria in Mice Overexpressing Renin. Int Heart J 59:1445-1453 (2018). PubMed: 30369568
    • Tiao MM  et al. Resveratrol ameliorates maternal and post-weaning high-fat diet-induced nonalcoholic fatty liver disease via renin-angiotensin system. Lipids Health Dis 17:178 (2018). PubMed: 30055626
    • Frantz EDC  et al. Exercise training modulates the hepatic renin-angiotensin system in fructose-fed rats. Exp Physiol 102:1208-1220 (2017). PubMed: 28626963
    View all Publications for this product

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question

    This is my enquiry about ab32213 and ab52174

    Read More
    Answer

    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

    ab52174 is antibody against a phosphorylated epitope so with this antibody it is best to block with BSA for sure instead of milk, as milk contains phosphorylated proteins which may bind the antibody, creating a high background. A recommended starting concentration for this antibody is 1:1000.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question


    Good noon. Thanks for your response, but actually I requested for AB32213 and AB52174 antibodies !!!!. Please apologize me regarding this.

    Can you send me quick reply.

    Read More
    Answer

    Thanks for your email.

    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Read More

    Question

    Good afternoon. Please apologize me once again with new antibodies enquiry. Could you please suggest me the correct primary diluents for these antibodies, we tried with 2% BSA for rat mesenteric artery and it didn’t show any band. I would be happy if you could provide standard western protocol mainly for blocking buffer and antibody diluents, since we have been using lot of primary antibodies from ABCAM.

    Thanks in advance and waiting for your reply.

    Read More
    Answer

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point for western blotting.

    A good blocking buffer we usually recommend is 5% BSA in TBST.

    A good antibody diluent to use is 1% BSA in TBST.

    Which antibody are you using to stain the rat mesenteric artery extracts?

    Read More

    Question

    send link to WB protocol and buffers

    Read More
    Answer

    Thank you for contacting us.

    The link to our list of buffer solutions and their composition is:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11414

    The link to our WB protocol is:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11375
    I have also attached the WB guide to this email for your convenience.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question


    Good afternoon. Thanks for your mail and prompt response with valuable information.

    Right now, I am having the antibody for ACE1 – ab11734 and ACE2- ab87436.

    Could you please suggest what kind of buffer and dilution range would be good for western blot applications.

    I am eagerly waiting for your response, I will try first with these antibodies, then I will decided what else I can do…

    Read More
    Answer

    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question


    I would be happy if someone would guide me to choose good antibody from your products for anti-Angiotensin Converting Enzyme 1 and 2 for Immunofluorescence and Western applications in Rat tissue.

    Since we tried lot of antibody with other sources, it wouldn’t give good result.

    Read More
    Answer

    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:
    https://www.abcam.com/Angiotensin-Converting-Enzyme-1-antibody-9B9-ab77990.html

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 2:
    https://www.abcam.com/Angiotensin-Converting-Enzyme-2-antibody-EPR4435-2-ab108252.html

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

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