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  1. Link

    ace2-antibody-epr44352-ab108252.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ACE2 antibody [EPR4435(2)] (ab108252)

  • Datasheet
  • SDS
Reviews (9)Q&A (6)References (73)

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Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Indirect ELISA - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
  • Anti-ACE2 antibody [EPR4435(2)] (ab108252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4435(2)] to ACE2
  • Suitable for: WB, IP, IHC-P, Indirect ELISA
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human ACE2 protein (ab151852)
Primary
Product image
Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)
Knockout
Product image
Human ACE2 knockout Caco-2 cell line (ab273731)

View more associated products

Overview

  • Product name

    Anti-ACE2 antibody [EPR4435(2)]
    See all ACE2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4435(2)] to ACE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Indirect ELISAmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab198988)

  • Positive control

    • WB: Human testis, kidney and lung tissue lysates; Human fetal kidney tissue lysate; Calu-3, HepG2 and Caco-2 cell lysates. IHC-P: Human, mouse, and rat kidney tissues. IP: Human testis tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4435(2)
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ACEs
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other

Associated products

  • Alternative Versions

    • Anti-ACE2 antibody [EPR4435(2)] - BSA and Azide free (ab239924)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • ACE2 peptide (ab198988)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ACE2 knockout Hep G2 cell line (ab273733)
  • KO cell lysates

    • Human ACE2 knockout Hep G2 cell lysate (ab275495)
  • Recombinant Protein

    • Recombinant Human ACE2 protein (ab151852)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with ACE2 peptide (ab198988).
IP
1/10 - 1/100.
IHC-P (1)
1/6400 - 1/32000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

Indirect ELISA
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with ACE2 peptide (ab198988).
IP
1/10 - 1/100.
IHC-P
1/6400 - 1/32000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

Indirect ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59272 Human
    • Entrez Gene: 70008 Mouse
    • Entrez Gene: 302668 Rat
    • Omim: 300335 Human
    • SwissProt: Q9BYF1 Human
    • SwissProt: Q8R0I0 Mouse
    • SwissProt: Q5EGZ1 Rat
    • Unigene: 178098 Human
    • Unigene: 13451 Mouse
    • Unigene: 129779 Rat
    see all
  • Alternative names

    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Images

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Wild-type HepG2 cell lysate
    Lane 2 : ACE2 knockout HepG2 cell lysate
    Lane 3 : Calu-3 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108252 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line ab273733 (knockout cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The section was incubated with ab108252 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Wild-type Caco-2 cell lysate
    Lane 2 : ACE2 knockout Caco-2 cell lysate
    Lane 3 : Calu-3 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108252 was shown to react with ACE2 in Caco-2 wild-type cells in western blot with loss of signal observed in ACE2 knockout cell line ab273731 (knockout cell lysate ab275516). Wild-type and ACE2 knockout Caco-2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Human testis cell lysate
    Lane 2 : Human kidney cell lysate
    Lane 3 : Human lung cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Caco-2 cell lysate
    Lane 6 : A549 cell lysate (negative control)

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green - ab108252 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.

    ab108252 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and Mouse anti Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Different batches of ab108252 were tested on Human kidney lysate at 0.2 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The section was incubated with ab108252 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling ACE2 with ab108252 at 1/6400 dilution. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Staining was visualised using Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The section was incubated with ab108252 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

    ab108252 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 0.6 μg primary antibody (1/20). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.

    Exposure time: 1 second.
    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.



    All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/500 dilution

    Lane 1 : Human testis tissue lysate at 10 µg
    Lane 2 : ab108252 + Human testis tissue lysate
    Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108252 in Human testis tissue lysate

    Observed band size: 110 kDa why is the actual band size different from the predicted?

  • Indirect ELISA - Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Indirect ELISA - Anti-ACE2 antibody [EPR4435(2)] (ab108252)

    ELISA using ab108252 at varying antibody concentrations (4000~0 ng/ml) and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Anti-ACE2 antibody [EPR4435(2)] (ab108252)
    Anti-ACE2 antibody [EPR4435(2)] (ab108252)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (73)

Publishing research using ab108252? Please let us know so that we can cite the reference in this datasheet.

ab108252 has been referenced in 73 publications.

  • Zhang Q  et al. Angiotensin-converting Enzyme 2-containing Small Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. Gastroenterology 160:958-961.e3 (2021). PubMed: 33022277
  • Moustaqil M  et al. SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species. Emerg Microbes Infect 10:178-195 (2021). PubMed: 33372854
  • Pedrosa MA  et al. Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19. Clin Sci (Lond) 135:465-481 (2021). PubMed: 33479758
  • Takahashi Y  et al. Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19. Sci Rep 11:3379 (2021). PubMed: 33564039
  • Zhao Y  et al. Type 2 diabetes mellitus impaired nasal immunity and increased the risk of hyposmia in COVID-19 mild pneumonia patients. Int Immunopharmacol 93:107406 (2021). PubMed: 33601246
View all Publications for this product

Customer reviews and Q&As

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1-10 of 15 Abreviews or Q&A

Western blot abreview for Anti-ACE2 antibody [EPR4435(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10-12.5% polyacrilamide gel Wet transfer at 400mA for 90 min.)
Loading amount
10 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 09 2022

Western blot abreview for Anti-ACE2 antibody [EPR4435(2)]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 30 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody [EPR4435(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High 9
Permeabilization
Yes - Tween 20 in Buffer
Specification
Kidney
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Mark Butters

Verified customer

Submitted Jan 21 2021

Western blot abreview for Anti-ACE2 antibody [EPR4435(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (primary bronchial epithelial cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
25 µg
Specification
primary bronchial epithelial cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 10 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4435(2)]

Poor
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Lung)
Permeabilization
Yes - triton-x
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
Fixative
Tried Methanol and Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Courtney Tindle

Verified customer

Submitted Aug 22 2020

Western blot abreview for Anti-ACE2 antibody [EPR4435(2)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 27 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4435(2)]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Jul 23 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4435(2)]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Alveolar Epithelial Cell)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Human Alveolar Epithelial Cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Jul 02 2020

Western blot abreview for Anti-ACE2 antibody [EPR4435(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Human Alveolar Epithelial Cell)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
30 µg
Specification
Human Alveolar Epithelial Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Jul 02 2020

Question

This is my enquiry about ab32213 and ab52174

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Abcam community

Verified customer

Asked on Jul 26 2012

Answer

Yes of course, thanks for your reply.

We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

ab52174 is antibody against a phosphorylated epitope so with this antibody it is best to block with BSA for sure instead of milk, as milk contains phosphorylated proteins which may bind the antibody, creating a high background. A recommended starting concentration for this antibody is 1:1000.

I hope this is helpful. Please contact me again if you have any further questions.

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Abcam Scientific Support

Answered on Jul 26 2012

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