Recombinant Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4435(2)] to ACE2
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ACE2 antibody [EPR4435(2)]
See all ACE2 primary antibodies -
Description
Rabbit monoclonal [EPR4435(2)] to ACE2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human ACE2 aa 200-300 (extracellular). The exact sequence is proprietary.
(Peptide available asab198988) -
Positive control
- WB: Human testis, kidney and lung tissue lysates; Human fetal kidney tissue lysate; Calu-3, HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and testis tissues. IP: Human testis tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4435(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.Can be blocked with ACE2 peptide (ab198988). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsN-glycosylation on Asn-90 may limit SARS infectivity. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all -
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Images
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![Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-448040-anti-ace2-antibody-epr44352-western-blot-hepg2-calu3-a549-human.jpg)
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1 : Wild-type HepG2 cell lysate
Lane 2 : ACE2 knockout HepG2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab108252 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108252 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line ab273733 (knockout cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-120764-anti-angiotensin-converting-enzyme-2-antibody-epr44352-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-391731-anti-ace2-antibody-epr4435-2-western-blot-human-testis-human-kidney-human-lung-hepg2.jpg)
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Human kidney cell lysate
Lane 3 : Human lung cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lane 6 : A549 cell lysate (negative control)
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab108252 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.
ab108252 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108252 and Mouse anti Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).
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![Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-120765-anti-angiotensin-converting-enzyme-2-antibody-epr44352-western-blot.jpg)
Western blot - Anti-ACE2 antibody [EPR4435(2)] (ab108252)All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 92 kDa -
![Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-389601-anti-ace2-antibody-epr44352-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ACE2 antibody [EPR4435(2)] (ab108252)ab108252 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 0.6 μg primary antibody (1/20). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.
Exposure time: 1 second.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes : Anti-ACE2 antibody [EPR4435(2)] (ab108252) at 1/500 dilution
Lane 1 : Human testis tissue lysate at 10 µg
Lane 2 : ab108252 + Human testis tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108252 in Human testis tissue lysate
Observed band size: 110 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-120763-anti-angiotensin-converting-enzyme-2-antibody-epr44352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (ab108252)ab108252, at 1/100 dilution, staining ACE2 in paraffin-embedded human testis tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-ACE2 antibody [EPR4435(2)] (ab108252)](https://www.abcam.com/ps/products/108/ab108252/Images/ab108252-7-benefits-of-recombinant-antibodies.png)
Anti-ACE2 antibody [EPR4435(2)] (ab108252)
Protocols
References (19)
ab108252 has been referenced in 19 publications.
- Smith JC et al. Cigarette Smoke Exposure and Inflammatory Signaling Increase the Expression of the SARS-CoV-2 Receptor ACE2 in the Respiratory Tract. Dev Cell 53:514-529.e3 (2020). PubMed: 32425701
- Song JJ et al. MicroRNA-122 aggravates angiotensin II-mediated apoptosis and autophagy imbalance in rat aortic adventitial fibroblasts via the modulation of SIRT6-elabela-ACE2 signaling. Eur J Pharmacol 883:173374 (2020). PubMed: 32682786
- Aguiar JA et al. Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue. Eur Respir J 56:N/A (2020). PubMed: 32675206
- Ding ZJ et al. Antihypertensive Activity of Eucommia Ulmoides Oliv: Male Flower Extract in Spontaneously Hypertensive Rats. Evid Based Complement Alternat Med 2020:6432173 (2020). PubMed: 32419815
- Lira DGD et al. Superimposing a high-fat diet on Schistosoma mansoni infection affects renin-angiotensin system components in the mouse kidney. Rev Soc Bras Med Trop 52:e20180371 (2019). PubMed: 30843967
