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Good afternoon. Please apologize me once again with new antibodies enquiry. Could you please suggest me the correct primary diluents for these antibodies, we tried with 2% BSA for rat mesenteric artery and it didn’t show any band. I would be happy if you could provide standard western protocol mainly for blocking buffer and antibody diluents, since we have been using lot of primary antibodies from ABCAM.
Thanks in advance and waiting for your reply.
Asked on Jul 24 2012
Thank you for your enquiry.
i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point for western blotting.
A good blocking buffer we usually recommend is 5% BSA in TBST.
A good antibody diluent to use is 1% BSA in TBST.
Which antibody are you using to stain the rat mesenteric artery extracts?
Answered on Jul 24 2012