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This is my enquiry about ab32213 and ab52174
Asked on Jul 26 2012
Yes of course, thanks for your reply.
We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.
ab52174 is antibody against a phosphorylated epitope so with this antibody it is best to block with BSA for sure instead of milk, as milk contains phosphorylated proteins which may bind the antibody, creating a high background. A recommended starting concentration for this antibody is 1:1000.
I hope this is helpful. Please contact me again if you have any further questions.
Answered on Jul 26 2012