Product nameAnti-ACE2 antibody [EPR4436]
See all ACE2 primary antibodies
DescriptionRabbit monoclonal [EPR4436] to ACE2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Human
Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact sequence is proprietary.
- WB: Human testis, kidney and lung lysates; Human fetal kidney lysates; HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and testis tissue. IP: Human testis tissue lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The use of an HRP/AP polymerized secondary antibody is recommended.
FunctionCarboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Tissue specificityExpressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
Sequence similaritiesBelongs to the peptidase M2 family.
modificationsN-glycosylation on Asn-90 may limit SARS infectivity.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Human kidney cell lysate
Lane 3 : Human lung cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lane 6 : A549 cell lysate (negative control)
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Lanes 1 - 6: Merged signal (red and green). Green - ab108209 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.
ab108209 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108209 and Mouse anti-Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
All lanes : Anti-ACE2 antibody [EPR4436] (ab108209)
Lane 1 : Human fetal kidney lysate
Lane 2 : Human testis lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
ab108209 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 2 μg primary antibody (1/30). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.
Exposure time: 1 second.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/500 dilution
Lane 1 : Human testis tissue lysate at 10 µg
Lane 2 : ab108209 + Human testis tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108209 in Human testis tissue lysate
Observed band size: 110 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
ab108209 has not yet been referenced specifically in any publications.