For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    ace2-antibody-epr4436-ab108209.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ACE2 antibody [EPR4436] (ab108209)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (9)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Immunoprecipitation - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)
  • Anti-ACE2 antibody [EPR4436] (ab108209)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4436] to ACE2
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-TMPRSS2 antibody [EPR3862] (ab109131)
Protein
Product image
Recombinant Human ACE2 protein (ab151852)
Knockout
Product image
Human ACE2 knockout Caco-2 cell line (ab273731)

View more associated products

Overview

  • Product name

    Anti-ACE2 antibody [EPR4436]
    See all ACE2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4436] to ACE2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis, kidney and lung lysates; Human fetal kidney lysates; Calu-3, HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and testis tissue. IP: Human testis tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4436
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ACEs
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other

Associated products

  • Alternative Versions

    • Anti-ACE2 antibody [EPR4436] - BSA and Azide free (ab247610)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ACE2 knockout Hep G2 cell line (ab273733)
  • KO cell lysates

    • Human ACE2 knockout Hep G2 cell lysate (ab275495)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The use of an HRP/AP polymerized secondary antibody is recommended.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The use of an HRP/AP polymerized secondary antibody is recommended.

Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59272 Human
    • Omim: 300335 Human
    • SwissProt: Q9BYF1 Human
    • Unigene: 178098 Human
    • Alternative names

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    Images

    • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

      Lane 1 : Wild-type HepG2 cell lysate
      Lane 2 : ACE2 knockout HepG2 cell lysate
      Lane 3 : Calu-3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 30 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab108209 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab108209 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line ab273733 (knockout cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108209 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

      Lane 1 : Wild-type Caco-2 cell lysate
      Lane 2 : ACE2 knockout Caco-2 cell lysate
      Lane 3 : Calu-3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 30 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab108209 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab108209 was shown to react with ACE2 in Caco-2 wild-type cells in western blot with loss of signal observed in ACE2 knockout cell line ab273731 (knockout cell lysate ab275516). Wild-type and ACE2 knockout Caco-2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108209 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

      Lane 1 : Human testis cell lysate
      Lane 2 : Human kidney cell lysate
      Lane 3 : Human lung cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Caco-2 cell lysate
      Lane 6 : A549 cell lysate (negative control)

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Lanes 1 - 6: Merged signal (red and green). Green - ab108209 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.

      ab108209 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108209 and Mouse anti-Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).

    • Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)
      All lanes : Anti-ACE2 antibody [EPR4436] (ab108209)

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 92 kDa
      Observed band size: 115 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-ACE2 antibody [EPR4436] (ab108209)
      Immunoprecipitation - Anti-ACE2 antibody [EPR4436] (ab108209)

      ab108209 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 2 μg primary antibody (1/30). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.

      Exposure time: 1 second.
      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.



      All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/500 dilution

      Lane 1 : Human testis tissue lysate at 10 µg
      Lane 2 : ab108209 + Human testis tissue lysate
      Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108209 in Human testis tissue lysate

      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Anti-ACE2 antibody [EPR4436] (ab108209)
      Anti-ACE2 antibody [EPR4436] (ab108209)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab108209? Please let us know so that we can cite the reference in this datasheet.

    ab108209 has been referenced in 9 publications.

    • Zhang Q  et al. Angiotensin-converting Enzyme 2-containing Small Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. Gastroenterology 160:958-961.e3 (2021). PubMed: 33022277
    • Huang KY  et al. Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Mol Med 13:e12828 (2021). PubMed: 33159417
    • Winstone H  et al. The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2. J Virol 95:N/A (2021). PubMed: 33563656
    • Fu Y & Xiong S Tagged extracellular vesicles with the RBD of the viral spike protein for delivery of antiviral agents against SARS-COV-2 infection. J Control Release 335:584-595 (2021). PubMed: 34089793
    • Rawle DJ  et al. ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactions. PLoS Pathog 17:e1009723 (2021). PubMed: 34214142
    • Mao Y  et al. The Adrenal Cortex, an Underestimated Site of SARS-CoV-2 Infection. Front Endocrinol (Lausanne) 11:593179 (2020). PubMed: 33488517
    • Liang Y  et al. Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model. Zool Res 41:621-631 (2020). PubMed: 33045777
    • Sun SH  et al. A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe 28:124-133.e4 (2020). PubMed: 32485164
    • Pellegrini L  et al. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27:951-961.e5 (2020). PubMed: 33113348

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-7 of 7 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 18 2020

    Western blot abreview for Anti-ACE2 antibody [EPR4436]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    40 µg
    Specification
    Mice hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 29 2020

    Western blot abreview for Anti-ACE2 antibody [EPR4436]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    30 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 27 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Alveolar Epithelial Cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Human Alveolar Epithelial Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Jul 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody [EPR4436]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 NaCitrate
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    In Hwa Um

    Verified customer

    Submitted Jun 26 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 21 2020

    Western blot abreview for Anti-ACE2 antibody [EPR4436]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Jerome Cattin

    Verified customer

    Submitted May 21 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.