Overview

  • Product name

    Acetate Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    < 20 µM
  • Assay time

    0h 50m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Acetate Assay Kit (Colorimetric) (ab204719) acetate is an assay where free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm. This kit is rapid, simple and sensitive. It can detect less than 20 µM free acetate in various samples.


    Acetate assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - incubate for 40 min
    - analyze with microplate reader

  • Notes

    Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Acetate Assay Buffer 1 x 27ml
    Acetate Enzyme Mix (Lyophilized) 1 vial
    Acetate Standard (Lyophilized) 1 vial
    Acetate Substrate Mix (Lyophilized) 1 vial
    ATP (Lyophilized) 1 vial
    Probe (Lyophilized) 1 vial

Images

  • Acetate Standard Curve.

  • Measurement of Acetate in human serum (5 μl), fish liver (~100 μg) and egg (~200 μg).

Protocols

References

This product has been referenced in:

  • Gronier B  et al. Increased cortical neuronal responses to NMDA and improved attentional set-shifting performance in rats following prebiotic (B-GOS®) ingestion. Eur Neuropsychopharmacol 28:211-224 (2018). Read more (PubMed: 29174530) »
  • Deobald D  et al. Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase. Sci Rep 8:7404 (2018). Read more (PubMed: 29743535) »
See all 3 Publications for this product

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