Key features and details
- Rabbit polyclonal to Acetyl Coenzyme A Carboxylase
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Acetyl Coenzyme A Carboxylase antibody
See all Acetyl Coenzyme A Carboxylase primary antibodies
DescriptionRabbit polyclonal to Acetyl Coenzyme A Carboxylase
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase aa 2333-2383 (C terminal).
Database link: NP_942131.1
- HeLa, 293T and NIH3T3 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab72046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).|
FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Involvement in diseaseAcetyl-CoA carboxylase 1 deficiency
Sequence similaritiesContains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1.
- Information by UniProt
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody (ab72046) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 266 kDa
Observed band size: 266 kDa
Additional bands at: 130 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab72046 has been referenced in 4 publications.
- Xu A et al. Diet-induced hepatic steatosis activates Ras to promote hepatocarcinogenesis via CPT1a. Cancer Lett 442:40-52 (2019). PubMed: 30401637
- Neves C et al. Dietary Glycotoxins Impair Hepatic Lipidemic Profile in Diet-Induced Obese Rats Causing Hepatic Oxidative Stress and Insulin Resistance. Oxid Med Cell Longev 2019:6362910 (2019). PubMed: 31341532
- Sujishi T et al. Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice. J Clin Biochem Nutr 57:244-53 (2015). WB . PubMed: 26566312
- Rawson P et al. Metabolic proteomics of the liver and mammary gland during lactation. J Proteomics 75:4429-35 (2012). WB ; Cow . PubMed: 22554911