Anti-Acetyl Coenzyme A Carboxylase antibody (ab72046)
Key features and details
- Rabbit polyclonal to Acetyl Coenzyme A Carboxylase
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Acetyl Coenzyme A Carboxylase antibody
See all Acetyl Coenzyme A Carboxylase primary antibodies -
Description
Rabbit polyclonal to Acetyl Coenzyme A Carboxylase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase aa 2300 to the C-terminus (C terminal).
Database link: Q13085 -
Positive control
- WB: HeLa, 293T and NIH/3T3 whole cell lysates. IHC-P: Human normal skin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa). |
Target
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Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
Involvement in disease
Acetyl-CoA carboxylase 1 deficiency -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 454602 Chimpanzee
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 100705 Mouse
- Entrez Gene: 107476 Mouse
- Entrez Gene: 397324 Pig
- Entrez Gene: 116719 Rat
- Entrez Gene: 60581 Rat
see all -
Alternative names
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
Images
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All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody (ab72046) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 266 kDa
Observed band size: 266 kDa
Additional bands at: 130 kDa, 90 kDa. We are unsure as to the identity of these extra bands. -
IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab72046 has been referenced in 9 publications.
- Wu Z et al. Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α. Cell Commun Signal 20:25 (2022). PubMed: 35248054
- Zhao G et al. Hydrolyzed Bound Phenolics from Rice Bran Alleviate Hyperlipidemia and Improve Gut Microbiota Dysbiosis in High-Fat-Diet Fed Mice. Nutrients 14:N/A (2022). PubMed: 35334934
- Zhang F et al. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway. Open Med (Wars) 17:365-374 (2022). PubMed: 35799598
- Wang J et al. Altered Liver Metabolism, Mitochondrial Function, Oxidative Status, and Inflammatory Response in Intrauterine Growth Restriction Piglets with Different Growth Patterns before Weaning. Metabolites 12:N/A (2022). PubMed: 36355136
- Ming Y et al. Interaction of Nuclear Receptor Subfamily 4 Group A Member 1 (Nr4a1) and Liver Linase B1 (LKB1) Mitigates Type 2 Diabetes Mellitus by Activating Monophosphate-Activated Protein Kinase (AMPK)/Sirtuin 1 (SIRT1) Axis and Inhibiting Nuclear Factor-kappa B (NF-?B) Activation. Med Sci Monit 26:e920278 (2020). PubMed: 31939452
- Xu A et al. Diet-induced hepatic steatosis activates Ras to promote hepatocarcinogenesis via CPT1a. Cancer Lett 442:40-52 (2019). PubMed: 30401637
- Neves C et al. Dietary Glycotoxins Impair Hepatic Lipidemic Profile in Diet-Induced Obese Rats Causing Hepatic Oxidative Stress and Insulin Resistance. Oxid Med Cell Longev 2019:6362910 (2019). PubMed: 31341532
- Sujishi T et al. Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice. J Clin Biochem Nutr 57:244-53 (2015). WB . PubMed: 26566312
- Rawson P et al. Metabolic proteomics of the liver and mammary gland during lactation. J Proteomics 75:4429-35 (2012). WB ; Cow . PubMed: 22554911