Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase antibody - C-terminal
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description

    Rabbit polyclonal to Acetyl Coenzyme A Carboxylase - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Acetyl Coenzyme A carboxylase alpha (C terminal). The exact sequence is proprietary.
    Database link: Q13085

  • Positive control

    • ICC/IF: HeLa cells. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Mouse cervix tissue; Rat liver tissue.

Applications

Our Abpromise guarantee covers the use of ab226891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 265 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody - C-terminal (ab226891) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 265 kDa



    5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Acetyl Coenzyme A carboxylase (green) using ab226891 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded mouse cervix tissue stained for Acetyl Coenzyme A carboxylase using ab226891 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat liver tissue stained for Acetyl Coenzyme A carboxylase using ab226891 at 1/500 dilution in immunohistochemical analysis.

References

ab226891 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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