Validated using a knockout cell line

Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)


  • Product name
    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase
  • Host species
  • Specificity
    Acetyl Coenzyme A Carboxylase is highly expressed in lipogenic tissues such as liver, adipose, and lactating mammary gland, and its activities are regulated at various levels [Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7515-20.].
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Acetyl Coenzyme A Carboxylase aa 350-450. The exact sequence is proprietary.
    (Peptide available as ab195232)

  • Positive control
    • A431 cell lysate, human liver tissue.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.


Our Abpromise guarantee covers the use of ab45174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificity
      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • Pathway
      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in disease
      Acetyl-CoA carboxylase 1 deficiency
    • Sequence similarities
      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all


    • All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate
      Lane 3 : Hela whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 265 kDa

      Lanes 1 - 4: Merged signal (red and green). Green - ab45174 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab45174 was shown to specifically react with Acetyl Coenzyme A Carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. Ab45174 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution + A431 cell lysate at 10 µg

      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Predicted band size: 265 kDa
      Observed band size: 265 kDa

    • Ab45174, at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Acetyl Coenzyme A Carboxylase with ab45174 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.



    This product has been referenced in:
    • Jeong SH  et al. Hippo-mediated suppression of IRS2/AKT signaling prevents hepatic steatosis and liver cancer. J Clin Invest 128:1010-1025 (2018). Read more (PubMed: 29400692) »
    • Meng X  et al. Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5'-Monophosphate (AMP)-activated Protein Kinase a1. J Biol Chem 291:10625-34 (2016). Read more (PubMed: 27013659) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Cell lysate - whole cell (C2C12 differentiated myotube)
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage Tris-Bis gel)
    Loading amount
    10 µg
    C2C12 differentiated myotube
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Aug 28 2017

    Thank you for your enquiry. I have contacted the originator of the product for the information you required. They have confirmed that the epitope region is indeed at 392-407 a.a. I hope this information will be helpful to you. If there is anythi...

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    Thank you for your enquiry. I can confirm that the band in the WB image is the ACC1 band. There is a 75% homology between ACC1 and ACC2. Generally, we say that if they share 85% or more, they will cross react, so I don’t think these will, but I can...

    Read More

    Thank you for your enquiry. An alignment of the peptide used to make ab45174 with human ACC2 ( shows 75% homology so there is a chance that ab45174 will cross react with ACC2. The antibody has only been test...

    Read More


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