Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Reviews (3)Q&A (3)References (27)
Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
  • Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies

  • Description

    Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase

  • Host species

    Rabbit

  • Specificity

    Acetyl Coenzyme A Carboxylase is highly expressed in lipogenic tissues such as liver, adipose, and lactating mammary gland, and its activities are regulated at various levels [Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7515-20.].

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Acetyl Coenzyme A Carboxylase aa 350-450. The exact sequence is proprietary.
    (Peptide available as ab195232)

  • Positive control

    • WB: HAP1, HeLa, C2C12 and A431 cell lysate; Rat adrenal gland tissue lysates IHC-P: Human liver and breast carcinoma tissue, Rat stomcah tissue, Mouse kidney tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).
IHC-P
1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

  • Tissue specificity

    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

  • Pathway

    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

  • Involvement in disease

    Acetyl-CoA carboxylase 1 deficiency

  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.

  • Post-translational
    modifications

    Phosphorylation on Ser-1263 is required for interaction with BRCA1.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACAC antibody
    • ACACA antibody
    • ACACA_HUMAN antibody
    • ACACB antibody
    • ACC alpha antibody
    • ACC antibody
    • ACC beta antibody
    • ACC-alpha antibody
    • ACC1 antibody
    • ACC2 antibody
    • ACCA antibody
    • ACCB antibody
    • Acetyl CoA carboxylase 1 antibody
    • Acetyl CoA carboxylase 2 antibody
    • Acetyl CoA carboxylase alpha antibody
    • Acetyl CoA carboxylase beta antibody
    • Acetyl Coenzyme A carboxylase alpha antibody
    • Acetyl Coenzyme A carboxylase beta antibody
    • Biotin carboxylase antibody
    • COA1 antibody
    • COA2 antibody
    • HACC275 antibody
    • OTTHUMP00000164069 antibody
    • OTTHUMP00000164070 antibody
    • OTTHUMP00000164076 antibody
    • OTTHUMP00000240532 antibody
    see all

Images

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution (unpurified)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 265 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab45174 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab45174 was shown to specifically react with Acetyl Coenzyme A Carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. Ab45174 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/1000 dilution (Purified)

    Lane 1 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 2 : Rat adrenal gland lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 265 kDa
    Observed band size: 265 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    We are unsure how to define the extra bands.

  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 265 kDa
    Observed band size: 265 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomcah tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Acetyl Coenzyme A Carboxylase with purified ab45174 at 1/400 dilution (2.06 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

    ab45174 (unpurified), at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue. Heat mediated antigen retrieval was perfromed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Acetyl Coenzyme A Carboxylase with ab45174 (unpurified) at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (27)

Publishing research using ab45174? Please let us know so that we can cite the reference in this datasheet.

ab45174 has been referenced in 27 publications.

  • Chen N  et al. Carbohydrate response element-binding protein regulates lipid metabolism via mTOR complex1 in diabetic nephropathy. J Cell Physiol 236:625-640 (2021). PubMed: 32583421
  • Ulisse V  et al. Regulation of axonal morphogenesis by the mitochondrial protein Efhd1. Life Sci Alliance 3:N/A (2020). PubMed: 32414840
  • Li M  et al. Sirt3 modulates fatty acid oxidation and attenuates cisplatin-induced AKI in mice. J Cell Mol Med 24:5109-5121 (2020). PubMed: 32281286
  • Lin Y  et al. Canagliflozin impairs blood reperfusion of ischaemic lower limb partially by inhibiting the retention and paracrine function of bone marrow derived mesenchymal stem cells. EBioMedicine 52:102637 (2020). PubMed: 31981975
  • Wang Q  et al. Role of endoplasmic reticulum stress and oxidative stress in vinyl chloride-induced hepatic steatosis in mice. Toxicol Appl Pharmacol 381:114730 (2019). PubMed: 31445928
View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Western blot abreview for Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Jul 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse hepatoma cell line)
Permeabilization
Yes - 0.5% Triton X-100
Specification
mouse hepatoma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 08 2019

Western blot abreview for Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 differentiated myotube)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage Tris-Bis gel)
Loading amount
10 µg
Specification
C2C12 differentiated myotube
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Aug 28 2017

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