Recombinant
RabMAb

Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] - BSA and Azide free (ab173584)

Overview

  • Product name
    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] - BSA and Azide free
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • General notes

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab173584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).

Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
  • Tissue specificity
    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
  • Pathway
    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
  • Involvement in disease
    Acetyl-CoA carboxylase 1 deficiency
  • Sequence similarities
    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-1263 is required for interaction with BRCA1.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAC antibody
    • ACACA antibody
    • ACACA_HUMAN antibody
    • ACACB antibody
    • ACC alpha antibody
    • ACC antibody
    • ACC beta antibody
    • ACC-alpha antibody
    • ACC1 antibody
    • ACC2 antibody
    • ACCA antibody
    • ACCB antibody
    • Acetyl CoA carboxylase 1 antibody
    • Acetyl CoA carboxylase 2 antibody
    • Acetyl CoA carboxylase alpha antibody
    • Acetyl CoA carboxylase beta antibody
    • Acetyl Coenzyme A carboxylase alpha antibody
    • Acetyl Coenzyme A carboxylase beta antibody
    • Biotin carboxylase antibody
    • COA1 antibody
    • COA2 antibody
    • HACC275 antibody
    • OTTHUMP00000164069 antibody
    • OTTHUMP00000164070 antibody
    • OTTHUMP00000164076 antibody
    • OTTHUMP00000240532 antibody
    see all

Images

  • All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ACACA (Acetyl Coenzyme A Carboxylase) HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 265 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab45174 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab45174 was shown to specifically react with Acetyl Coenzyme A Carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. Ab45174 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45174).

References

ab173584 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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