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    acetyl-coenzyme-a-carboxylase-antibody-epr4971-ab109368.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

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Submit a review Q&A (2)References (2)

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Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
  • Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A Carboxylase
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description

    Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A Carboxylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, and SH-SY5Y cell lysates. Human brain tissue and Human skeletal muscle tissue. 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4971
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab203994)
    • Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] - BSA and Azide free (ab231681)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Predicted molecular weight: 266 kDa.

For unpurified use at 1/1000- 1/10000.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Predicted molecular weight: 266 kDa.

For unpurified use at 1/1000- 1/10000.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IP.

Target

  • Function

    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
  • Tissue specificity

    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
  • Pathway

    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
  • Involvement in disease

    Acetyl-CoA carboxylase 1 deficiency
  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-1263 is required for interaction with BRCA1.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13085 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 31 Human
    • Entrez Gene: 32 Human
    • Omim: 200350 Human
    • Omim: 601557 Human
    • SwissProt: O00763 Human
    • SwissProt: Q13085 Human
    • Unigene: 160556 Human
    • Unigene: 234898 Human
    • Alternative names

      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Images

    • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate
      Lane 3 : Hela whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 266 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab109368 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab109368 was shown to specifically react with Acetyl Coenzyme A carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. Ab109368 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Immunocytochemistry/ Immunofluorescence analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Acetyl Coenzyme A carboxylase with Purified ab109368 at 1:250 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Acetyl Coenzyme A carboxylase with purified ab109368 at 1/100 dilution (5 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cells without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Acetyl Coenzyme A carboxylase with purified  ab109368 at 1:500 dilution (1.05 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Flow Cytometry (Intracellular) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Overlay histogram showing SH-SY5Y cells stained with unpurified ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 266 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Immunofluorescent staining of 293 cells using unpurified ab109368 at 1/100 dilution

    • Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/5000 dilution

      Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 266 kDa
      Observed band size: 266 kDa



      5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using unpurified ab109368 at 1/250 dilution.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

      Immunohistochemical analysis of paraffin-embedded brain tissue using unpurified ab109368 at 1/250 dilution.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)
      Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab109368? Please let us know so that we can cite the reference in this datasheet.

    ab109368 has been referenced in 2 publications.

    • Wang L  et al. Adropin inhibits the phenotypic modulation and proliferation of vascular smooth muscle cells during neointimal hyperplasia by activating the AMPK/ACC signaling pathway. Exp Ther Med 21:560 (2021). PubMed: 33850532
    • Hu PA  et al. Bromelain Confers Protection against the Non-Alcoholic Fatty Liver Disease in Male C57bl/6 Mice. Nutrients 12:N/A (2020). PubMed: 32443556

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    ReviewID: 30779 AbID: 109368, Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] Rating: Excellent Sample: Species: Mouse Type: Tissue sections Specification: Adult brain Application: Application: Fixative: Formaldehyde Antigen retrieval step: None Permeabilization: No Blocking step: Serum as blocking agent for 3 hours at 25°C Other detail: Dilution: 1/1000 Incubation time: 24 hours at 25°C Diluent: 2% normal goat serum in PBS Secondary Antibody: Name: Non-Abcam Antibody was used: Jax Conjugation: Cy3 ® Dilution: 1/1000 Additional Data: Additional Notes: Brains were were perfused with 4% PFA prior to slicing. Biotin visualization also worked well.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2012

    Answer

    Thank you for participating in our 100% Abreview Testing Discount program. On17 July 2012 your review was returned with a request for any images which you may have relating to your testing of ab109368 in IHC-FoFr on mouse brain sections. We have not as yet heard back from you regarding this. Images are extremely helpful to other researchers when choosing a product, if you have any that you could add to your review you will gain another XXX Abpoints which may be redeemed towards products or gift cards. If you do not have any images or are unable to add these at this time we ask that you please re-submit Abreview #XXXX so that we may publish it in the current form. Thank you very much for your help in this and for testing this product. Please let us know if you have any questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 28 2012

    Question

    Please send a testing discount for IHC on frozen mouse brain.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 29 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test ab109368 in IHC on frozen mouse brain sections. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC on frozen tissue (IHC-Fr) and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome.

    Read More

    Abcam Scientific Support

    Answered on Mar 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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