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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
  • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
  • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)

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Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description

    Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293 and HAP1 cell lysates.
  • General notes

    Ab173583 is the carrier-free version of ab68191. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab173583 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1885Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Acetyl Coenzyme A Carboxylase protein (ab196428)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
Notes
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificity

      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • Pathway

      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in disease

      Acetyl-CoA carboxylase 1 deficiency
    • Sequence similarities

      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      modifications

      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q13085 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 31 Human
      • Entrez Gene: 32 Human
      • Entrez Gene: 100705 Mouse
      • Entrez Gene: 107476 Mouse
      • Entrez Gene: 116719 Rat
      • Entrez Gene: 60581 Rat
      • Omim: 200350 Human
      • Omim: 601557 Human
      • SwissProt: O00763 Human
      • SwissProt: Q13085 Human
      • SwissProt: O00763 Mouse
      • SwissProt: Q5SWU9 Mouse
      • SwissProt: O70151 Rat
      • SwissProt: P11497 Rat
      • Unigene: 160556 Human
      • Unigene: 234898 Human
      • Unigene: 31374 Mouse
      • Unigene: 163753 Rat
      • Unigene: 217177 Rat
      • Unigene: 44372 Rat
      see all
    • Alternative names

      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Images

    • Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
      Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
      All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : ACACA knockout HAP1 whole cell lysate
      Lane 3 : HEK293 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 277 kDa
      Observed band size: 266 kDa why is the actual band size different from the predicted?



      Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

    • Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
      Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

      Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).

    • Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
      Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab173583? Please let us know so that we can cite the reference in this datasheet.

    ab173583 has not yet been referenced specifically in any publications.

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