Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
![Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)](https://www.abcam.com/ps/products/173/ab173583/Images/ab173583-368366-anti-acetyl-coenzyme-a-carboxylase-phospho-s79-antibody-ep1885y-westernblot-knockout-hap1-empty-acaca-ko-human-lysates.jpg "Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free
- Suitable for: Dot blot, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free
See all Acetyl Coenzyme A Carboxylase primary antibodies -
Description
Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293 and HAP1 cell lysates.
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General notes
Ab173583 is the carrier-free version of ab68191. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab173583 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1885Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
|
| Notes |
|---|
|
Dot blot
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa). |
Target
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Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
Involvement in disease
Acetyl-CoA carboxylase 1 deficiency -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 100705 Mouse
- Entrez Gene: 107476 Mouse
- Entrez Gene: 116719 Rat
- Entrez Gene: 60581 Rat
- Omim: 200350 Human
- Omim: 601557 Human
see all -
Alternative names
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
Images
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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ACACA knockout HAP1 whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 277 kDa
Observed band size: 266 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. Ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).
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![Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)](https://www.abcam.com/ps/products/173/ab173583/Images/ab173583-1-ab68191253315ab68191dot.jpg)
Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68191).
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![Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)](https://www.abcam.com/ps/products/173/ab173583/Images/ab173583-3-benefits-of-recombinant-antibodies.png)
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] - BSA and Azide free (ab173583)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab173583 has not yet been referenced specifically in any publications.