Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270] (ab222774)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM270] to Acetyl Coenzyme A Carboxylase (phospho S79)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270]
See all Acetyl Coenzyme A Carboxylase primary antibodies -
Description
Rabbit monoclonal [RM270] to Acetyl Coenzyme A Carboxylase (phospho S79) -
Host species
Rabbit -
Specificity
ab222774 reacts with human Acetyl CoA Carboxylase only when phosphorylated at Ser79. There is no cross-reactivity with Acetyl CoA Carboxylase without phosphorylation at Ser79. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase (phospho S79).
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Positive control
- WB: A431 cell lysate. IHC-P: Human breast cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab222774 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM270 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 266 kDa.
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IHC-P |
1/1000 - 1/2000.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 266 kDa. |
IHC-P
1/1000 - 1/2000. |
Target
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Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
Involvement in disease
Acetyl-CoA carboxylase 1 deficiency -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Omim: 200350 Human
- Omim: 601557 Human
- SwissProt: O00763 Human
- SwissProt: Q13085 Human
- Unigene: 160556 Human
- Unigene: 234898 Human
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Alternative names
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
Images
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Acetyl Coenzyme A Carboxylase (phospho S79) with ab222774 at 1/5000 dilution in immunohistochemical analysis.
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All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270] (ab222774) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate treated with lambda protein phosphatase
Predicted band size: 266 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab222774 has been referenced in 1 publication.
- Zhao T et al. TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling. Exp Ther Med 22:1271 (2021). PubMed: 34594408