Recombinant

Recombinant Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270] (ab222774)

Overview

  • Product name

    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description

    Rabbit monoclonal [RM270] to Acetyl Coenzyme A Carboxylase (phospho S79)
  • Host species

    Rabbit
  • Specificity

    ab222774 reacts with human Acetyl CoA Carboxylase only when phosphorylated at Ser79. There is no cross-reactivity with Acetyl CoA Carboxylase without phosphorylation at Ser79.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase (phospho S79).

  • Positive control

    • WB: A431 cell lysate. IHC-P: Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab222774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 266 kDa.
IHC-P 1/1000 - 1/2000.

Target

  • Function

    Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
  • Tissue specificity

    Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
  • Pathway

    Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
  • Involvement in disease

    Acetyl-CoA carboxylase 1 deficiency
  • Sequence similarities

    Contains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-1263 is required for interaction with BRCA1.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAC antibody
    • ACACA antibody
    • ACACA_HUMAN antibody
    • ACACB antibody
    • ACC alpha antibody
    • ACC antibody
    • ACC beta antibody
    • ACC-alpha antibody
    • ACC1 antibody
    • ACC2 antibody
    • ACCA antibody
    • ACCB antibody
    • Acetyl CoA carboxylase 1 antibody
    • Acetyl CoA carboxylase 2 antibody
    • Acetyl CoA carboxylase alpha antibody
    • Acetyl CoA carboxylase beta antibody
    • Acetyl Coenzyme A carboxylase alpha antibody
    • Acetyl Coenzyme A carboxylase beta antibody
    • Biotin carboxylase antibody
    • COA1 antibody
    • COA2 antibody
    • HACC275 antibody
    • OTTHUMP00000164069 antibody
    • OTTHUMP00000164070 antibody
    • OTTHUMP00000164076 antibody
    • OTTHUMP00000240532 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Acetyl Coenzyme A Carboxylase (phospho S79) with ab222774 at 1/5000 dilution in immunohistochemical analysis.

  • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [RM270] (ab222774) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate treated with lambda protein phosphatase

    Predicted band size: 266 kDa

References

ab222774 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222774.
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