• Product name
    Anti-acetyl Lysine antibody [1C6]
    See all acetyl Lysine primary antibodies
  • Description
    Mouse monoclonal [1C6] to acetyl Lysine
  • Host species
  • Specificity
    ab22550 recognises proteins with acetylated lysine.
  • Tested applications
    Suitable for: IP, ChIP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3

  • Positive control
    • HeLa cell lysate. MCF7 cell line.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab22550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 3 µg/ml.
ChIP Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/2000.


  • Relevance
    In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names
    • pan acetyl Lysine antibody


  • Immunoflouroescence analysis of HeLa Cells labelling lysine acetylated proteins with ab22550. Formalin fixed cells were permeabalized with 0.1& Triton X-100 in TBS for 10 mins at room temperature and subsequently blocked with BSA at room temperature for 15 mins. Cells were then probed with ab22550 at 1/100 for 1 hour at room temperature. The secondary used was a DyLight® 488 goat anti-mouse used at 1/400 for 30 minutes at room temperature. Additional counterstains used were F-actin with a DyLight® 554 Phalloidin and Neuclei stained using a Hoechst 33342 conjugate. Image was taken at X20 magnification.

  • Chromatin Co-Immunoprecipitation (ChIP) analysis  using ab22550 binding acetylated lysines in 10E+06 LNCaP cells. Protein binding was detected using real-time PCR.

    Positive control: Fold enrichment of ab22550.
    Negative Control: Non-specific IgG.

  • ICC/IF image of ab22550 stained MCF7 cells. The cells were 4% formaldehye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Saidi D  et al. Glioma-induced SIRT1-dependent activation of hMOF histone H4 lysine 16 acetyltransferase in microglia promotes a tumor supporting phenotype. Oncoimmunology 7:e1382790 (2018). Read more (PubMed: 29308302) »
  • Utani K  et al. Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Nucleic Acids Res 45:7807-7824 (2017). Read more (PubMed: 28549174) »
See all 5 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Mouse Cell lysate - whole cell (primary hepatoctyes)
Negative control
mouse IgG
primary hepatoctyes
Detection step
Real-time PCR
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control

Abcam user community

Verified customer

Submitted Dec 15 2014

Mouse Cell lysate - whole cell (primary hepatoctyes)
Total protein in input
20 µg
Immuno-precipitation step
Protein A
primary hepatoctyes

Abcam user community

Verified customer

Submitted Dec 15 2014

Western blot
Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions
Non-reduced Denaturing (15)
Loading amount
30 µg
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 11 2014


Thank you very much for your interest in ab22550. To our knowledge, ab22550 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab22550 now, test it in IPand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab22550 in IP. I will then send a discount code. This code must be issued before purchasing ab22550 so please wait for my reply before ordering. 2. Purchase ab22550 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab22550 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Jurkat cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
10 µg
trichostatin A 250 nM treated cells for 24h
Jurkat cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 19 2010


Thank you for your enquiry. The synthetic peptide used to raise this antibody was a peptide sequence surrounding the acetylated lysine 9 of histone H3. This antibody has been shown to generate a ladder in a HeLa whole cell lysate demonstrating that it has the potential to recognise acetylated, non-histone proteins. I cannot guarantee that it will recognise every acetyl-lysine epitope given that each epitope will vary dependent on the adjacent residues. May I suggest that you consider our monoclonal N-epsilon acetyl Lysine antibody [11A1] (ab409). Mike Schutkowski has left a favourable review following it application against various acetylated lysine peptides. Please do not hesitate to contact me should you require further assistance.

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Thank you for your enquiry. Yes, by Western blot, this antibody detects a ladder of acetylated proteins in HeLa cell lysates. In immunofluorescence procedures, this antibody recognizes acetylated lysine in gamma irradiated HeLa cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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