Overview

  • Product name

    Anti-acetyl Lysine antibody [7F8]
    See all acetyl Lysine primary antibodies
  • Description

    Mouse monoclonal [7F8] to acetyl Lysine
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Acetylated KLH

  • Positive control

    • IHC-P: Mouse backskin tissue. IHC-Fr: Mouse backskin tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    7F8
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names

    • pan acetyl Lysine antibody

Images

  • Paraffin-embedded mouse backskin tissue stained for acetyl Lysine using ab61384 at 1/100 dilution in immunohistochemical analysis.

    Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 for 1 hour at room temperature.

  • ab61384 staining acetyl lysine in mouse back skin tissue.

  • Lanes 1-2 : Anti-acetyl Lysine antibody [7F8] (ab61384) at 1/1000 dilution


    Lane 1 : acetylated BSA at 0.75 µg
    Lane 2 : non-acetylated BSA at 0.75 µg
    Lane 3 : marker

References

This product has been referenced in:

  • Kutil Z  et al. The unraveling of substrate specificity of histone deacetylase 6 domains using acetylome peptide microarrays and peptide libraries. FASEB J 33:4035-4045 (2019). Read more (PubMed: 30496698) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
100000 cells
Specification
HCT116
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 03 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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