Key features and details
- Mouse monoclonal [7F8] to acetyl Lysine
- Suitable for: IHC-P, IHC-Fr, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-acetyl Lysine antibody [7F8]
See all acetyl Lysine primary antibodies
DescriptionMouse monoclonal [7F8] to acetyl Lysine
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ELISAmore details
Species reactivityReacts with: Species independent
- IHC-P: Mouse backskin tissue. IHC-Fr: Mouse backskin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab61384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
- pan acetyl Lysine antibody
Paraffin-embedded mouse backskin tissue stained for acetyl Lysine using ab61384 at 1/100 dilution in immunohistochemical analysis.
Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 for 1 hour at room temperature.
ab61384 staining acetyl lysine in mouse back skin tissue.
Lanes 1-2 : Anti-acetyl Lysine antibody [7F8] (ab61384) at 1/1000 dilution
Lane 1 : acetylated BSA at 0.75 µg
Lane 2 : non-acetylated BSA at 0.75 µg
Lane 3 : marker
ab61384 has been referenced in 2 publications.
- Kutil Z et al. The unraveling of substrate specificity of histone deacetylase 6 domains using acetylome peptide microarrays and peptide libraries. FASEB J 33:4035-4045 (2019). PubMed: 30496698
- Farkas M & McMahon SB Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling. Methods Mol Biol 1983:255-262 (2019). PubMed: 31087303