Anti-acetyl Lysine antibody (ab80178)
Key features and details
- Rabbit polyclonal to acetyl Lysine
- Suitable for: WB, IP, ELISA, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-acetyl Lysine antibody
See all acetyl Lysine primary antibodies -
Description
Rabbit polyclonal to acetyl Lysine -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ELISA, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Acetylated KLH Conjugates
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Positive control
- TSA treated mouse spleen cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Corresponding Antibody
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF | (5) |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/100. (see Abreview)
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Notes |
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WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/100. (see Abreview) |
Target
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Relevance
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo. -
Alternative names
- pan acetyl Lysine antibody
Images
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Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A FITC conjugated Goat Anti-Rabbit secondary antibody (green) was used at 1/200 dilution. Localization: Nucleus and Cytoplasm.
(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.
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ab80178 staining acetyl Lysine in human HepG2 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 2% serum for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/150 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence analysis of Heat Shocked HeLa Cells labeling acetyl Lysine with ab80178 at 1/100 dilution. Cells were fixed with 2% Formaldehyde for 20 min at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. A R-PE conjugated Goat Anti-Rabbit secondary antibody (yellow) was used at 1/200 dilution. Localization: Nucleus and Cytoplasm.
(A) DAPI (blue) nuclear stain. (B) Anti-acetyl Lysine antibody (ab80178) (C) Composite.
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Anti-acetyl Lysine antibody (ab80178) + Cell lysates prepared from TSA treated mouse spleen cells
Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Primary Antibody: Rabbit Anti-Acetyl Lysine Polyclonal Antibody (ab80178) at 1:1000.
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ab80178 staining acetyl Lysine in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.
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ab80178 staining acetyl Lysine (green) in the neuromuscular junction of fruit fly cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in formaldehde, permeabilized using 0.4% Triton-X, blocked with 10% NGS for 1 hour at 20°C, then incubated with ab80178 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab80178 has been referenced in 41 publications.
- Siqueira E et al. Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome. Mol Ther Nucleic Acids 27:621-644 (2022). PubMed: 35036070
- Chen IP et al. Viral E protein neutralizes BET protein-mediated post-entry antagonism of SARS-CoV-2. Cell Rep 40:111088 (2022). PubMed: 35839775
- Huegel J et al. CUDC907, a dual phosphoinositide-3 kinase/histone deacetylase inhibitor, promotes apoptosis of NF2 Schwannoma cells. Oncotarget 13:890-904 (2022). PubMed: 35875610
- Malikova AZ et al. Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors. Int J Mol Sci 22:N/A (2021). PubMed: 33925399
- Bianchi A et al. Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis. J Immunol 206:904-916 (2021). PubMed: 33441438