• Product name
    Anti-acetyl Lysine antibody (HRP)
    See all acetyl Lysine primary antibodies
  • Description
    Rabbit polyclonal to acetyl Lysine (HRP)
  • Host species
  • Conjugation
  • Specificity
    This antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Acetylated KLH conjugates.

  • General notes

    The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.00
    Constituents: 0.268% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab23364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Microarray: Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 3 kDa.


  • Relevance
    In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names
    • pan acetyl Lysine antibody


This product has been referenced in:
  • van Gent R  et al. SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner. PLoS One 9:e98438 (2014). WB ; Human . Read more (PubMed: 24875183) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry. I am sorry to hear that you have been having problems with ab23364 anti-acetyl Lysine. Quality is important to Abcam, if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement if the antibody was delivered within the past 90 days. I have examined your complaint and I have a few comments. It is very difficult for me to comment on the performance of our antibody when compared to a competitor as I am unaware of the degree of cross reactivity the antibody may demonstrate against non-acetylated proteins or the number of acetylated proteins that it may recognise by western blot. Even if the immunising peptide was the same this is likely to vary from antiserum to antiserum. You mentioned acetylated histones so I am assuming that these are your targets of interest. Please can I ask whether you have tried acid extracting histones with and without 5mM Sodium Butyrate treatment before performing western blotting. This will enable you to determine whether the increase in acetylation is detected by this antibody. To control this experiment it is essential the loadings are equalised by a parallel Coomassie stained histone prep or examination of the Ponceau transfer. Abcam has an excellent histone extraction protocol located at the link below. http://marketing.abcam.com/index.html?pageconfig=view_protocol&pid=316 Should you feel that the antibody is still not performing after considering my comments please submit a technical questionnaire by clicking on the link below. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=23364&mode=questionaire Please do not hesitate to contact me should you require further assistance.

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