Recombinant Anti-acetyl Lysine antibody [RM101] (ab190479)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM101] to acetyl Lysine
- Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IF
- Reacts with: Species independent
Overview
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Product name
Anti-acetyl Lysine antibody [RM101]
See all acetyl Lysine primary antibodies -
Description
Rabbit monoclonal [RM101] to acetyl Lysine -
Host species
Rabbit -
Specificity
ab190479 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, or lysine with other modification. -
Tested applications
Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to acetyl Lysine conjugated to bovine serum albumin.
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Positive control
- A431 cells treated with Trichostatin A; HeLa whole cell lysate - Trichostatin A treated
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM101 -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/2000.
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IHC-P |
1/100 - 1/500.
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ChIP |
1/100 - 1/500.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/500.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. |
IHC-P
1/100 - 1/500. |
ChIP
1/100 - 1/500. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/100 - 1/500. |
ICC/IF
1/100 - 1/500. |
Target
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Relevance
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo. -
Alternative names
- pan acetyl Lysine antibody
Images
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Lane 1: A431 whole cell lysate
Lane 2: A431 whole cell lysate (pretreated with Trichostatin A)
Lane 3: A431 whole cell lysate immunoprecipitated with Rabbit IgG
Lane 4: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with Rabbit IgG
Lane 5: A431 whole cell lysate immunoprecipitated with ab190479 at 1/500
Lane 6: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with ab190479 at 1/500Western blot performed using anti-PTEN mouse monoclonal antibody.
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Immunocytochemical staining of HeLa cells labelling Acetyl Lysine with ab190479 at 1:100. Actin filaments are labelled using fluorescein phalloidin (green), and nuclei are stained with DAPI (blue).
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All lanes : Anti-acetyl Lysine antibody [RM101] (ab190479) at 1/2000 dilution
Lanes 1 & 3 & 5 : Lysate of nontreated HeLa cells
Lanes 2 & 4 & 6 : Lysate of HeLa cells treated with Trichostatin A
Developed using the ECL technique.Exposure time increased from blot on left (lanes 1, 2) to blot on right (lanes 5,6).
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Immunofluorescent analysis of A431cells nontreated (left) or treated with Trichostatin A (right), using ab190479 at 1/500 followed by a PE conjugated secondary antibody (red) and DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab190479 has been referenced in 19 publications.
- Lin JX et al. m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer. Cell Death Dis 13:463 (2022). PubMed: 35568711
- Hao K et al. Nicotinamide reverses deficits in puberty-born neurons and cognitive function after maternal separation. J Neuroinflammation 19:232 (2022). PubMed: 36131290
- Tao Z et al. Sirt1 coordinates with ERa to regulate autophagy and adiposity. Cell Death Discov 7:53 (2021). PubMed: 33723227
- Wang ZH et al. Nicotinamide Riboside Enhances Endothelial Precursor Cell Function to Promote Refractory Wound Healing Through Mediating the Sirt1/AMPK Pathway. Front Pharmacol 12:671563 (2021). PubMed: 34054544
- Ni Y et al. Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase. Cancer Sci 112:4075-4086 (2021). PubMed: 34310804