For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    acetylcholinesterase-antibody-ab31276.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Acetylcholine
Share by email

Anti-Acetylcholinesterase antibody (ab31276)

  • Datasheet
Reviews (4)Q&A (3)References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Acetylcholinesterase antibody (ab31276)
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)

Key features and details

  • Goat polyclonal to Acetylcholinesterase
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Biochemical
Product image
PD 98059, MEK1 inhibitor (ab120234)
Assay
Product image
Sphingomyelinase Assay Kit (Colorimetric) (ab138876)
Protein
Product image
Recombinant Human Acetylcholinesterase protein (ab157830)

View more associated products

Overview

  • Product name

    Anti-Acetylcholinesterase antibody
    See all Acetylcholinesterase primary antibodies
  • Description

    Goat polyclonal to Acetylcholinesterase
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    QFDHYSKQDRCSDL

    , corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Acetylcholine
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias

Associated products

  • Assay kits

    • Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
    • Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Acetylcholinesterase protein (ab157830)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Rat
Rabbit
Cow
Cat
Application Abreviews Notes
WB (3)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

1 hour primary incubation is recommended for this product.

Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Function

    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
  • Tissue specificity

    Isoform H is highly expressed in erythrocytes.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization

    Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Target information above from: UniProt accession P22303 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 540446 Cow
    • Entrez Gene: 43 Human
    • Entrez Gene: 83817 Rat
    • Omim: 100740 Human
    • SwissProt: P23795 Cow
    • SwissProt: P22303 Human
    • SwissProt: Q29499 Rabbit
    • SwissProt: P37136 Rat
    • Unigene: 154495 Human
    • Unigene: 105879 Rat
    see all
  • Alternative names

    • ACEE antibody
    • ACES_HUMAN antibody
    • Acetylcholinesterase antibody
    • AChE antibody
    • Apoptosis related acetylcholinesterase antibody
    • ARACHE antibody
    • N ACHE antibody
    • N-ACHE antibody
    • YT antibody
    • YT blood group antibody
    see all

Images

  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)This image is courtesy of an Abreview submitted by Ender Avci
    All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution

    Lane 1 : SkHep1 cell lysate
    Lane 2 : Snu387 cell lysate
    Lane 3 : SW620 cell lysate
    Lane 4 : Zebrafish brain lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa


    Exposure time: 20 minutes

    See Abreview

  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)

    Predicted band size: 68 kDa
    Observed band size: 60-70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).

    Predicted band size: 68 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.
    Detected by chemiluminescence.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (10)

    Publishing research using ab31276? Please let us know so that we can cite the reference in this datasheet.

    ab31276 has been referenced in 10 publications.

    • Yekta R  et al. The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism. Int J Biol Macromol 160:35-46 (2020). PubMed: 32454110
    • Price D  et al. Humanin Blocks the Aggregation of Amyloid-ß Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3. Biochemistry 59:1981-2002 (2020). PubMed: 32383868
    • Price D  et al. IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner. Sci Rep 10:5083 (2020). PubMed: 32193421
    • Liao Z  et al. Acetylcholinesterase is not a generic marker of extracellular vesicles. J Extracell Vesicles 8:1628592 (2019). PubMed: 31303981
    • Campanari ML  et al. Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients. J Alzheimers Dis 53:831-41 (2016). PubMed: 27258420
    • Vetreno RP  et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . PubMed: 25405505
    • García-Ayllón MS  et al. Readthrough acetylcholinesterase is increased in human liver cirrhosis. PLoS One 7:e44598 (2012). WB ; Human . PubMed: 23028565
    • García-Ayllón MS  et al. Altered levels of acetylcholinesterase in Alzheimer plasma. PLoS One 5:e8701 (2010). WB ; Human . PubMed: 20090844
    • Silveyra MX  et al. Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation. Mol Cell Biol 28:2908-19 (2008). WB ; Human . PubMed: 18299393
    • García-Ayllón MS  et al. Cerebrospinal fluid acetylcholinesterase changes after treatment with donepezil in patients with Alzheimer's disease. J Neurochem 101:1701-11 (2007). PubMed: 17326766

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Acetylcholinesterase antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (using simple western blot system, WES)
    Loading amount
    3 µg
    Specification
    Brain
    Blocking step
    Milk-free Antibody Diluent (protein simple, 043-524) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2019

    Western blot abreview for Anti-Acetylcholinesterase antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-20%)
    Sample
    Mouse Tissue lysate - whole (Hippocampus)
    Specification
    Hippocampus
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 02 2014

    Western blot abreview for Anti-Acetylcholinesterase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Zebrafish Tissue lysate - other (zebrafish brain)
    Specification
    zebrafish brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Ender Avci

    Verified customer

    Submitted Jul 08 2013

    ELISA abreview for Anti-Acetylcholinesterase antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Purified protein (human acetylcholinesterase from erythrocytes)
    Specification
    human acetylcholinesterase from erythrocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 29 2013

    Question

    . I want to order an ACHE
    antibody from Abcam but I find a few and could not decide on the one to be
    ordered. Attached I am sending you my shortlist, I don't have the epitope
    information of all, could you please align the epitope info that you have
    with zebrafish ACHE protein? And maybe then you can advice one of them
    depending on the quality of the alignment. In addition I would like to ask
    you for a test code. After selecting the antibody I will order, test and
    write an abreview. Could you please send a test code?
    I would be glad if you could help.
    Best wishes,
    Ender

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2013

    Answer

    Thank you for your email.

    I have done a homology search between human ACHE and Danio Rerio (Zebrafish). The data shows intermittent homology, please check

    http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20130419-114224-0217-79716166-oy

    As you can see homology in C-terminal end is quite good so antibody ab31276 could be suitable producthttps://www.abcam.com/acetylcholinesterase-antibody-ab31276.html . If you are interested in using this product I am happy to provide you Abbtrial offer. Please check the terms and conditions;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    I hope this information will helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2013

    Question

    Hello,

    Is it possible to help her ?

    Regards


    Dear Team,

    I have been looking for the whole cDNA sequence of Acetylcholinesterase(AChE). In particular, I am looking for the isoform of AChE which is expressed in brain (AChE - isoform T) (ref:http://www.uniprot.org/uniprot/P22303#P22303). Kindly let me know if you can provide with the cDNA sequence for the isoform - T that I mentioned above.Also, if you can, pls. provide me the whole sequnce and let me know the details, the minimum possible amount of sample you could provide and its cost asap.

    Kindly do the needful at the latest.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you for your enquiry.

    I am sorry to confirm I am unable to find the cDNA sequence of acetylcholinesterase isoform T. We do have a link to the Unigene protein database site which has information regarding isoform T:

    http://www.uniprot.org/uniprot/P22303#P22303

    I'm sorry it is not clear if you are requesting a sample of the antibody, or a sample of the cDNA? Regrettably, we do not sell cDNAsamples. With regards to samples of antibody, I am sorry to confirm that because we carry over 80,000 products, it is unfortunately notfeasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I am sorry we have not been able to provide as much information and help as we would like on this occasion. However, I hope the information is useful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Question

    I want to order Acetylcholinesterase antibody (ab31276), as you know, the AchE antibody may be cross-react wtih butyrlcholinesterase. I just wonder if Acetylcholinesterase antibody (ab31276) also cross-react with butyrlcholinesterase. thank you!

    Read More

    Abcam community

    Verified customer

    Asked on Feb 18 2008

    Answer

    Thank you for your enquiry. I have enquired at the originator of the product and according to their immunizing peptide sequence blast result, there is no match with butyrylcholinesterase. Therefore, this product should not cross react with butyrlcholinesterase. I hope this information will helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Feb 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.