Anti-Acetylcholinesterase antibody (ab31276)
Key features and details
- Goat polyclonal to Acetylcholinesterase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Acetylcholinesterase antibody
See all Acetylcholinesterase primary antibodies -
Description
Goat polyclonal to Acetylcholinesterase -
Host species
Goat -
Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide:
QFDHYSKQDRCSDL
, corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase -
Positive control
- Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
|
All applications |
Rat
Rabbit
Cow
Cat
|
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
Tissue specificity
Isoform H is highly expressed in erythrocytes. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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Database links
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: Q29499 Rabbit
- SwissProt: P37136 Rat
see all -
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
Images
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Western blot - Anti-Acetylcholinesterase antibody (ab31276)This image is courtesy of an Abreview submitted by Ender AvciAll lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1 : SkHep1 cell lysate
Lane 2 : Snu387 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Zebrafish brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20 minutes
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Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.Detected by chemiluminescence.
Datasheets and documents
References (10)
ab31276 has been referenced in 10 publications.
- Yekta R et al. The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism. Int J Biol Macromol 160:35-46 (2020). PubMed: 32454110
- Price D et al. Humanin Blocks the Aggregation of Amyloid-ß Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3. Biochemistry 59:1981-2002 (2020). PubMed: 32383868
- Price D et al. IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner. Sci Rep 10:5083 (2020). PubMed: 32193421
- Liao Z et al. Acetylcholinesterase is not a generic marker of extracellular vesicles. J Extracell Vesicles 8:1628592 (2019). PubMed: 31303981
- Campanari ML et al. Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients. J Alzheimers Dis 53:831-41 (2016). PubMed: 27258420
- Vetreno RP et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . PubMed: 25405505
- García-Ayllón MS et al. Readthrough acetylcholinesterase is increased in human liver cirrhosis. PLoS One 7:e44598 (2012). WB ; Human . PubMed: 23028565
- García-Ayllón MS et al. Altered levels of acetylcholinesterase in Alzheimer plasma. PLoS One 5:e8701 (2010). WB ; Human . PubMed: 20090844
- Silveyra MX et al. Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation. Mol Cell Biol 28:2908-19 (2008). WB ; Human . PubMed: 18299393
- García-Ayllón MS et al. Cerebrospinal fluid acetylcholinesterase changes after treatment with donepezil in patients with Alzheimer's disease. J Neurochem 101:1701-11 (2007). PubMed: 17326766