- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Acetylcholinesterase antibody
See all Acetylcholinesterase primary antibodies -
DescriptionGoat polyclonal to Acetylcholinesterase
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Host speciesGoat
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SpecificityThis antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Cat
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Immunogen
Synthetic peptide:
QFDHYSKQDRCSDL
, corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase -
Positive control
- Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab31276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
Target
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FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
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Tissue specificityIsoform H is highly expressed in erythrocytes.
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Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
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Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
- Information by UniProt
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Database links
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 11423 Mouse
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: P21836 Mouse
see all -
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
Images
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Western blot - Anti-Acetylcholinesterase antibody (ab31276)This image is courtesy of an Abreview submitted by Ender AvciAll lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1 : SkHep1 cell lysate
Lane 2 : Snu387 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Zebrafish brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20 minutes
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Western blot - Anti-Acetylcholinesterase antibody (ab31276)Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 60-70 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-Acetylcholinesterase antibody (ab31276)Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).
Predicted band size: 68 kDa
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.Detected by chemiluminescence.
Protocols
References
This product has been referenced in:
- Vetreno RP et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . Read more (PubMed: 25405505) »
- García-Ayllón MS et al. Readthrough acetylcholinesterase is increased in human liver cirrhosis. PLoS One 7:e44598 (2012). WB ; Human . Read more (PubMed: 23028565) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"