Anti-Acetylcholinesterase antibody (ab31276)

Reviews (4)Q&A (3)References (10)
Western blot - Anti-Acetylcholinesterase antibody (ab31276)
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)

Key features and details

  • Goat polyclonal to Acetylcholinesterase
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Acetylcholinesterase antibody
    See all Acetylcholinesterase primary antibodies

  • Description

    Goat polyclonal to Acetylcholinesterase

  • Host species

    Goat

  • Specificity

    This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide:

    QFDHYSKQDRCSDL

    , corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Rat
Rabbit
Cow
Cat
Application Abreviews Notes
WB (3)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

1 hour primary incubation is recommended for this product.
Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

1 hour primary incubation is recommended for this product.

Target

Images

  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)This image is courtesy of an Abreview submitted by Ender Avci
    All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution

    Lane 1 : SkHep1 cell lysate
    Lane 2 : Snu387 cell lysate
    Lane 3 : SW620 cell lysate
    Lane 4 : Zebrafish brain lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa


    Exposure time: 20 minutes

    See Abreview

  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)

    Predicted band size: 68 kDa
    Observed band size: 60-70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Western blot - Anti-Acetylcholinesterase antibody (ab31276)
    Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).

    Predicted band size: 68 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.
    Detected by chemiluminescence.

References (10)

Publishing research using ab31276? Please let us know so that we can cite the reference in this datasheet.

ab31276 has been referenced in 10 publications.

  • Yekta R  et al. The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism. Int J Biol Macromol 160:35-46 (2020). PubMed: 32454110
  • Price D  et al. Humanin Blocks the Aggregation of Amyloid-ß Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3. Biochemistry 59:1981-2002 (2020). PubMed: 32383868
  • Price D  et al. IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner. Sci Rep 10:5083 (2020). PubMed: 32193421
  • Liao Z  et al. Acetylcholinesterase is not a generic marker of extracellular vesicles. J Extracell Vesicles 8:1628592 (2019). PubMed: 31303981
  • Campanari ML  et al. Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients. J Alzheimers Dis 53:831-41 (2016). PubMed: 27258420
  • Vetreno RP  et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . PubMed: 25405505
  • García-Ayllón MS  et al. Readthrough acetylcholinesterase is increased in human liver cirrhosis. PLoS One 7:e44598 (2012). WB ; Human . PubMed: 23028565
  • García-Ayllón MS  et al. Altered levels of acetylcholinesterase in Alzheimer plasma. PLoS One 5:e8701 (2010). WB ; Human . PubMed: 20090844
  • Silveyra MX  et al. Presenilin 1 interacts with acetylcholinesterase and alters its enzymatic activity and glycosylation. Mol Cell Biol 28:2908-19 (2008). WB ; Human . PubMed: 18299393
  • García-Ayllón MS  et al. Cerebrospinal fluid acetylcholinesterase changes after treatment with donepezil in patients with Alzheimer's disease. J Neurochem 101:1701-11 (2007). PubMed: 17326766

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Acetylcholinesterase antibody

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (using simple western blot system, WES)
Loading amount
3 µg
Specification
Brain
Blocking step
Milk-free Antibody Diluent (protein simple, 043-524) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Oct 28 2019

Western blot abreview for Anti-Acetylcholinesterase antibody

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Sample
Mouse Tissue lysate - whole (Hippocampus)
Specification
Hippocampus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 02 2014

Western blot abreview for Anti-Acetylcholinesterase antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Zebrafish Tissue lysate - other (zebrafish brain)
Specification
zebrafish brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Ender Avci

Verified customer

Submitted Jul 08 2013

ELISA abreview for Anti-Acetylcholinesterase antibody

 Inconclusive
Abreviews
Application
ELISA
Sample
Human Purified protein (human acetylcholinesterase from erythrocytes)
Specification
human acetylcholinesterase from erythrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Direct
Read More

Abcam user community

Verified customer

Submitted Apr 29 2013

Question

. I want to order an ACHE
antibody from Abcam but I find a few and could not decide on the one to be
ordered. Attached I am sending you my shortlist, I don't have the epitope
information of all, could you please align the epitope info that you have
with zebrafish ACHE protein? And maybe then you can advice one of them
depending on the quality of the alignment. In addition I would like to ask
you for a test code. After selecting the antibody I will order, test and
write an abreview. Could you please send a test code?
I would be glad if you could help.
Best wishes,
Ender

Read More
Answer

Thank you for your email.

I have done a homology search between human ACHE and Danio Rerio (Zebrafish). The data shows intermittent homology, please check

http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20130419-114224-0217-79716166-oy

As you can see homology in C-terminal end is quite good so antibody ab31276 could be suitable producthttps://www.abcam.com/acetylcholinesterase-antibody-ab31276.html . If you are interested in using this product I am happy to provide you Abbtrial offer. Please check the terms and conditions;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information will helpful. Should you have any question please do not hesitate to contact me.

Read More

Question

Hello,

Is it possible to help her ?

Regards


Dear Team,

I have been looking for the whole cDNA sequence of Acetylcholinesterase(AChE). In particular, I am looking for the isoform of AChE which is expressed in brain (AChE - isoform T) (ref:http://www.uniprot.org/uniprot/P22303#P22303). Kindly let me know if you can provide with the cDNA sequence for the isoform - T that I mentioned above.Also, if you can, pls. provide me the whole sequnce and let me know the details, the minimum possible amount of sample you could provide and its cost asap.

Kindly do the needful at the latest.

Read More
Answer

Thank you for your enquiry.

I am sorry to confirm I am unable to find the cDNA sequence of acetylcholinesterase isoform T. We do have a link to the Unigene protein database site which has information regarding isoform T:

http://www.uniprot.org/uniprot/P22303#P22303

I'm sorry it is not clear if you are requesting a sample of the antibody, or a sample of the cDNA? Regrettably, we do not sell cDNAsamples. With regards to samples of antibody, I am sorry to confirm that because we carry over 80,000 products, it is unfortunately notfeasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I am sorry we have not been able to provide as much information and help as we would like on this occasion. However, I hope the information is useful. Please do not hesitate to contact us again with any other questions.

Read More

Question

I want to order Acetylcholinesterase antibody (ab31276), as you know, the AchE antibody may be cross-react wtih butyrlcholinesterase. I just wonder if Acetylcholinesterase antibody (ab31276) also cross-react with butyrlcholinesterase. thank you!

Read More
Answer

Thank you for your enquiry. I have enquired at the originator of the product and according to their immunizing peptide sequence blast result, there is no match with butyrylcholinesterase. Therefore, this product should not cross react with butyrlcholinesterase. I hope this information will helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

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