Overview

  • Product name
    Anti-Acetylcholinesterase antibody
    See all Acetylcholinesterase primary antibodies
  • Description
    Goat polyclonal to Acetylcholinesterase
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Cat
  • Immunogen

    Synthetic peptide:

    QFDHYSKQDRCSDL

    , corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase

  • Positive control
    • Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

Target

Images

  • All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution

    Lane 1 : SkHep1 cell lysate
    Lane 2 : Snu387 cell lysate
    Lane 3 : SW620 cell lysate
    Lane 4 : Zebrafish brain lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa


    Exposure time: 20 minutes

    See Abreview

  • Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)

    Predicted band size: 68 kDa
    Observed band size: 60-70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).

    Predicted band size: 68 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.
    Detected by chemiluminescence.

References

This product has been referenced in:
  • Campanari ML  et al. Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients. J Alzheimers Dis 53:831-41 (2016). Read more (PubMed: 27258420) »
  • Vetreno RP  et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . Read more (PubMed: 25405505) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Sample
Mouse Tissue lysate - whole (Hippocampus)
Specification
Hippocampus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 02 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Zebrafish Tissue lysate - other (zebrafish brain)
Specification
zebrafish brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ender Avci

Verified customer

Submitted Jul 08 2013

Application
ELISA
Sample
Human Purified protein (human acetylcholinesterase from erythrocytes)
Specification
human acetylcholinesterase from erythrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Direct

Abcam user community

Verified customer

Submitted Apr 29 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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