Western blot abreview for Anti-Acetylcholinesterase antibody

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Zebrafish Tissue lysate - other (zebrafish brain)
Specification
zebrafish brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk in TBS-T
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit polyclonal to goat IgG H&L (HRP)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Bands
Specific: 60 kDa Non-specific: 170 kDa
Exposure
20 minute(s) and 0 second(s)
Positive control
Human hepatocellular carcinoma cell lines: SKHep1, Snu387 Human colon cancer cell line: SW620

Additional data

Additional Notes
PVDF membrane, NP40 lysis buffer with 1X protease inhibitor cocktail used.
Abcam response
Thank you for including human positive controls in your blot of samples of zebrafish, a previously untested species.

Dr. Ender Avci

Verified customer

Submitted Jul 08 2013

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