Overview

  • Product name

    Anti-Acetylcholinesterase antibody
    See all Acetylcholinesterase primary antibodies
  • Description

    Rabbit polyclonal to Acetylcholinesterase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Acetylcholinesterase aa 551-614.
    Database link: P22303

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell IHC-P: Human hippocampus FFPE tissue sections.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 20% Glycerol, 2.68% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
  • Tissue specificity

    Isoform H is highly expressed in erythrocytes.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization

    Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACEE antibody
    • ACES_HUMAN antibody
    • Acetylcholinesterase antibody
    • AChE antibody
    • Apoptosis related acetylcholinesterase antibody
    • ARACHE antibody
    • N ACHE antibody
    • N-ACHE antibody
    • YT antibody
    • YT blood group antibody
    see all

Images

  • All lanes : Anti-Acetylcholinesterase antibody (ab97298) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Paraffin-embedded mouse fore brain stained with ab97298 dilution: 1:250.

  • ab97298, at a 1/200 dilution, staining Acetylcholinesterase in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
    Lower image: merged with DNA probe.

References

This product has been referenced in:

  • Holmes CJ  et al. Burn Injury Alters Epidermal Cholinergic Mediators and Increases HMGB1 and Caspase 3 in Autologous Donor Skin and Burn Margin. Shock 47:175-183 (2017). Read more (PubMed: 27648692) »
See 1 Publication for this product

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