Key features and details
- Goat polyclonal to Acetylcholinesterase (Biotin)
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot
- Reacts with: Cow
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Acetylcholinesterase antibody (Biotin)
See all Acetylcholinesterase primary antibodies
DescriptionGoat polyclonal to Acetylcholinesterase (Biotin)
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
Species reactivityReacts with: Cow
Acetylcholinesterase from Bovine Erythrocytes.
10-20 Biotin molecules per goat IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and Protease free
Concentration information loading...
Purification notesThis product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
Our Abpromise guarantee covers the use of ab34533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000 - 1/2000.
This product has been assayed against 1.0µg of Acetylcholinesterase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
Suitable for other antibody based assays using streptavidin or avidin comjugates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificityIsoform H is highly expressed in erythrocytes.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
- Information by UniProt
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab34533 has been referenced in 1 publication.
- Freitas Leal JK et al. Acetylcholinesterase provides new insights into red blood cell ageing in vivo and in vitro. Blood Transfus 15:232-238 (2017). PubMed: 28518050