Name: Anti-Acetylcholinesterase antibody [EPR18978]
Brand: RabMAb
SKU: ab183591

Product name

Anti-Acetylcholinesterase antibody [EPR18978]
See all Acetylcholinesterase primary antibodies
Description

Rabbit monoclonal [EPR18978] to Acetylcholinesterase
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Synthetic peptide within Mouse Acetylcholinesterase aa 50-150. The exact sequence is proprietary.
Database link: P21836
Positive control
- WB: Mouse brain and striatum lysates; rat striatum, hippocampus and brain lysates. IHC-P: Mouse striatum and skeletal muscle tissues; rat striatum and skeletal muscle tissues. IHC-Fr: Mouse brain (Coronal section) and rat brain (sagittal section) tissues.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18978
Isotype

IgG
Research areas

Alternative Versions
- Anti-Acetylcholinesterase antibody [EPR18978] - BSA and Azide free (ab240274)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Acetylcholinesterase protein (ab157830)

Our Abpromise guarantee covers the use of ab183591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P		1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr		1/1000. Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20)

Function

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificity

Isoform H is highly expressed in erythrocytes.
Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.
Cellular localization

Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
Target information above from: UniProt accession P22303 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 11423 Mouse
- Entrez Gene: 83817 Rat
- SwissProt: P21836 Mouse
- SwissProt: P37136 Rat
- Unigene: 255464 Mouse
- Unigene: 105879 Rat
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
- AChE antibody
- Apoptosis related acetylcholinesterase antibody
- ARACHE antibody
- N ACHE antibody
- N-ACHE antibody
- YT antibody
- YT blood group antibody
see all

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse striatum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 30 seconds, Lane 2: 3 minutes.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution
Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1 : Rat striatum lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Immunohistochemistry protocols
Western blot protocols

Datasheet

ab183591 has not yet been referenced specifically in any publications.

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Recombinant Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

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