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RecombinantRabMAb

Recombinant Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Submit a review Submit a question References (6)
Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
  • Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18978] to Acetylcholinesterase
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Acetylcholinesterase antibody [EPR18978]
    See all Acetylcholinesterase primary antibodies

  • Description

    Rabbit monoclonal [EPR18978] to Acetylcholinesterase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and striatum lysates; rat striatum, hippocampus and brain lysates. IHC-P: Mouse striatum and skeletal muscle tissues; rat striatum and skeletal muscle tissues. IHC-Fr: Mouse brain (Coronal section) and rat brain (sagittal section) tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18978

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/1000.

Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20)
Notes
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/1000.

Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20)

Target

  • Function

    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

  • Tissue specificity

    Isoform H is highly expressed in erythrocytes.

  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.

  • Cellular localization

    Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Information by UniProt

  • Database links

    • Alternative names

      • ACEE antibody
      • ACES_HUMAN antibody
      • Acetylcholinesterase antibody
      • AChE antibody
      • Apoptosis related acetylcholinesterase antibody
      • ARACHE antibody
      • N ACHE antibody
      • N-ACHE antibody
      • YT antibody
      • YT blood group antibody
      see all

    Images

    • Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse striatum lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 30 seconds, Lane 2: 3 minutes.

      Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).

    • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution

    • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

      Lane 1 : Rat striatum lysate
      Lane 2 : Rat hippocampus lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

      Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
      Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    References (6)

    Publishing research using ab183591? Please let us know so that we can cite the reference in this datasheet.

    ab183591 has been referenced in 6 publications.

    • Liu B  et al. Lemon essential oil ameliorates age-associated cognitive dysfunction via modulating hippocampal synaptic density and inhibiting acetylcholinesterase. Aging (Albany NY) 12:8622-8639 (2020). PubMed: 32392535
    • Hour FQ  et al. Magnetic targeted delivery of the SPIONs-labeled mesenchymal stem cells derived from human Wharton's jelly in Alzheimer's rat models. J Control Release 321:430-441 (2020). PubMed: 32097673
    • Yao K  et al. MicroRNA-9 mediated the protective effect of ferulic acid on hypoxic-ischemic brain damage in neonatal rats. PLoS One 15:e0228825 (2020). PubMed: 32470970
    • Jimenez Naranjo C  et al. Effect of cannabidiol on muscarinic neurotransmission in the pre-frontal cortex and hippocampus of the poly I:C rat model of schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry 94:109640 (2019). PubMed: 31108177
    • Yue J  et al. MicroRNA-187 Reduces Acute Ischemic Renal Podocyte Injury via Targeting Acetylcholinesterase. J Surg Res 244:302-311 (2019). PubMed: 31302329
    • Riquelme R  et al. Role of ovarian sympathetic nerves and cholinergic local system during cold stress. J Endocrinol 242:115-124 (2019). PubMed: 31176305

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