Recombinant Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

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Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18978] to Acetylcholinesterase
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat

Carrier Free

Product name

Anti-Acetylcholinesterase antibody [EPR18978]
See all Acetylcholinesterase primary antibodies
Description

Rabbit monoclonal [EPR18978] to Acetylcholinesterase
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain and striatum lysates; rat striatum, hippocampus and brain lysates. IHC-P: Mouse striatum and skeletal muscle tissues; rat striatum and skeletal muscle tissues. IHC-Fr: Mouse brain (Coronal section) and rat brain (sagittal section) tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18978
Isotype

IgG
Research areas

Alternative Versions
- Anti-Acetylcholinesterase antibody [EPR18978] - BSA and Azide free (ab240274)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Acetylcholinesterase protein (ab157830)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P		1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr		1/1000. Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20)

Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/1000. Antigen retrieval step: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20)

Function

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificity

Isoform H is highly expressed in erythrocytes.
Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.
Cellular localization

Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
Target information above from: UniProt accession P22303 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 11423 Mouse
- Entrez Gene: 83817 Rat
- SwissProt: P21836 Mouse
- SwissProt: P37136 Rat
- Unigene: 255464 Mouse
- Unigene: 105879 Rat
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
- AChE antibody
- Apoptosis related acetylcholinesterase antibody
- ARACHE antibody
- N ACHE antibody
- N-ACHE antibody
- YT antibody
- YT blood group antibody
see all

Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse striatum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 30 seconds, Lane 2: 3 minutes.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution
Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

Lane 1 : Rat striatum lysate
Lane 2 : Rat hippocampus lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

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Publishing research using ab183591? Please let us know so that we can cite the reference in this datasheet.

ab183591 has been referenced in 5 publications.

Liu B et al. Lemon essential oil ameliorates age-associated cognitive dysfunction via modulating hippocampal synaptic density and inhibiting acetylcholinesterase. Aging (Albany NY) 12:8622-8639 (2020). PubMed: 32392535
Hour FQ et al. Magnetic targeted delivery of the SPIONs-labeled mesenchymal stem cells derived from human Wharton's jelly in Alzheimer's rat models. J Control Release 321:430-441 (2020). PubMed: 32097673
Yao K et al. MicroRNA-9 mediated the protective effect of ferulic acid on hypoxic-ischemic brain damage in neonatal rats. PLoS One 15:e0228825 (2020). PubMed: 32470970
Yue J et al. MicroRNA-187 Reduces Acute Ischemic Renal Podocyte Injury via Targeting Acetylcholinesterase. J Surg Res 244:302-311 (2019). PubMed: 31302329
Riquelme R et al. Role of ovarian sympathetic nerves and cholinergic local system during cold stress. J Endocrinol 242:115-124 (2019). PubMed: 31176305

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (5)

Customer reviews and Q&As