Recombinant Anti-Acetylcholinesterase antibody [EPR23268-202] - BSA and Azide free (ab274388)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23268-202] to Acetylcholinesterase - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Acetylcholinesterase antibody [EPR23268-202] - BSA and Azide free
See all Acetylcholinesterase primary antibodies -
Description
Rabbit monoclonal [EPR23268-202] to Acetylcholinesterase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain tissue lysate; Mouse and rat brain and striatum tissue lysates; HeLa, PC-12 and NIH/3T3 whole cell lysates. IP: Human and mouse brain tissue lysate.
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General notes
ab274388 is the carrier-free version of ab253201.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23268-202 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab274388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
Tissue specificity
Isoform H is highly expressed in erythrocytes. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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Database links
- Entrez Gene: 43 Human
- Entrez Gene: 11423 Mouse
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P22303 Human
- SwissProt: P21836 Mouse
- SwissProt: P37136 Rat
- Unigene: 154495 Human
see all -
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
Images
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All lanes : Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse striatum tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat striatum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human liver (PMID: 2400605).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253201).
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Acetylcholinesterase was immunoprecipitated from 0.35 mg human brain tissue lysate with ab253201 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human brain tissue lysate 10µg.
Lane 2: ab253201 IP in human brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253201 in human brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253201).
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All lanes : Anti-Acetylcholinesterase antibody [EPR23268-202] (ab253201) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 59 seconds; Lane 2: 26 seconds; Lane 3: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253201).
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Acetylcholinesterase was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab253201 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10µg.
Lane 2: ab253201 IP in mouse brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253201 in mouse brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253201).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab274388 has not yet been referenced specifically in any publications.