Anti-Acetylcholinesterase antibody [HR2] (ab2803)

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Overview

  • Product name

    Anti-Acetylcholinesterase antibody [HR2]
    See all Acetylcholinesterase primary antibodies

  • Description

    Mouse monoclonal [HR2] to Acetylcholinesterase

  • Host species

    Mouse

  • Specificity

    This antibody does not detect butyrylcholinesterase (BChE).

  • Tested applications

    Suitable for: ELISA, IHC-Fr, IP, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rabbit, Guinea pig, Cow, Cat, Human, Macaque monkey
    Predicted to work with: Non human primatesDoes not react with: Rat, Amphibian

  • Immunogen

    Full length protein corresponding to Human Acetylcholinesterase. Purified Human cerebellar acetylcholinesterase.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    HR2

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.
  • Application notes
    Is unsuitable for WB.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
    • Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

      Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in HeLa cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

      Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in Neuro-2a cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody [HR2] (ab2803)

      Immunocytochemistry/Immunofluorescence analysis of Acetylcholinesterase shows staining in U251 cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2803 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human Rectum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Acetylcholinesterase (ab2803) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
    • Flow Cytometry - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Flow Cytometry - Anti-Acetylcholinesterase antibody [HR2] (ab2803)
      Overlay histogram showing HeLa cells stained with ab2803 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2803, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:

    • Dafferner AJ  et al. Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure. Chem Res Toxicol 30:1897-1910 (2017). Read more (PubMed: 28892361) »
    • Yu ZG  et al. Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model. Evid Based Complement Alternat Med 2016:7074563 (2016). Read more (PubMed: 27190536) »
    See all 6 Publications for this product

    Publishing research using ab2803? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    ANTIBODY CODE ab2803 BATCH NUMBER 46727 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM We would like to to do a peptide block as a control to show the specificity of ab2803 in monkey brain tissue. Do you offer the original peptide for which the antibody was made? For purchase? SAMPLE rhesus monkey brain PRIMARY ANTIBODY ab2803 SECONDARY ANTIBODY vector ba-2000 DETECTION METHOD DAB POSITIVE AND NEGATIVE CONTROLS USED no primary ANTIBODY STORAGE CONDITIONS 4c FIXATION OF SAMPLE paraformaldehyde ANTIGEN RETRIEVAL none PERMEABILIZATION STEP triton BLOCKING CONDITIONS serum from secondary HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? floating sections are processed in nets

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    Answer

    Thank you for your enquiry. The immunogen used to generate this antibody is not available. To our knowledge ab2803 has not previously been tested in monkey tissue nor in paraformaldehyde fixed tissue. How is ab2803 working for you? My suggestion is to run a control with one of the tested species - Human, Rabbit, Cow, Cat and Guinea pig. If you have any more questions, please contact us again.

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    Question

    I would like to know if you have already tested this antibody in flow cytometry? If not, do you know another one that could work with FACS.

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    Answer

    Thank you for your enquiry. To our knowledge, ab2803 has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. The other Acetylcholinesterase antibodies that we currently have - ab2802, ab7396, ab7397 - have also not yet been tested in FACS.

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