Question (8663) | Anti-Acetylcholinesterase antibody [HR2] (ab2803)

Go to datasheet (ab2803)

Question

ANTIBODY CODE ab2803 BATCH NUMBER 46727 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM We would like to to do a peptide block as a control to show the specificity of ab2803 in monkey brain tissue. Do you offer the original peptide for which the antibody was made? For purchase? SAMPLE rhesus monkey brain PRIMARY ANTIBODY ab2803 SECONDARY ANTIBODY vector ba-2000 DETECTION METHOD DAB POSITIVE AND NEGATIVE CONTROLS USED no primary ANTIBODY STORAGE CONDITIONS 4c FIXATION OF SAMPLE paraformaldehyde ANTIGEN RETRIEVAL none PERMEABILIZATION STEP triton BLOCKING CONDITIONS serum from secondary HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? floating sections are processed in nets

Answer

Thank you for your enquiry. The immunogen used to generate this antibody is not available. To our knowledge ab2803 has not previously been tested in monkey tissue nor in paraformaldehyde fixed tissue. How is ab2803 working for you? My suggestion is to run a control with one of the tested species - Human, Rabbit, Cow, Cat and Guinea pig. If you have any more questions, please contact us again.

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