Overview

  • Product name
    Acetylcholinesterase Assay Kit (Fluorometric - Green)
    See all Acetylcholinesterase kits
  • Detection method
    Fluorescent
  • Sample type
    Cell culture extracts, Whole Blood
  • Assay type
    Enzyme activity
  • Sensitivity
    0.1 mU/ml
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Acetylcholinesterase Assay KIt (Fluorometric - Green) (ab138872) uses an outstanding Thiol Green Indicator to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. Thiol Green Indicator is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument. The fluorescence intensity of Thiol Green Indicator is used to measure AChE activity. Compared to the existing thiol probes (e.g., mBBr and bBBr), Thiol Green Indicator is much more sensitive.


    This product is an optimized "mix-and-read" assay that provides an ultrasensitive fluorometric one-step protocol to detect as little as 0.01mU AChE in a 100 µl assay volume (0.1 mU/ml). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. 


     


    Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.


     


    If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure red fluorescnce (Ex/Em = 540/590 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Red) (ab138873).

  • Notes

    Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer’s disease (AD) and myasthenia gravis.

  • Platform
    Microplate reader

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    Acetylcholinesterase Standard 1 vial
    Acetylthiocholine 1 vial
    Assay Buffer 1 x 25ml
    DMSO 1 x 100µl
    Thiol Green Indicator 1 vial
  • Research areas
  • Function
    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
  • Tissue specificity
    Isoform H is highly expressed in erythrocytes.
  • Sequence similarities
    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization
    Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Information by UniProt
  • Alternative names
    • ACEE
    • ACES_HUMAN
    • Acetylcholinesterase
    • AChE
    • Apoptosis related acetylcholinesterase
    • ARACHE
    • N ACHE
    • N-ACHE
    • YT
    • YT blood group
    see all

Images

  • Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138872 using a fluorescence microplate reader. As low as 0.01 mU/well of acetylcholinesterase can be detected with 20 minutes incubation (n=3).

Protocols

References

ab138872 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

You can use 50 mM Na phosphate buffer, pH 6.5.

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