Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate, Plasma, Serum
- Sensitivity: 0.1 mU/ml
Product nameAcetylcholinesterase Assay Kit (Fluorometric -Red)
See all Acetylcholinesterase kits
Sample typeSerum, Plasma, Cell Lysate
Assay typeEnzyme activity
Assay time0h 20m
Species reactivityReacts with: Mammals, Other species
Acetylcholinesterase Assay Kit (Fluorometric -Red) ( ab138873) uses a red indicator to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions.
The assay can be used for monitoring and quantifying AChE activity in blood, cell extracts or other solutions. The fluorescence intensity of the indicator is proportional to the amount of choline formed, which is proportional to the AChE activity.
The assay is an optimized “mix and read” assay using a simple one-step fluorometric protocol to detect as little as 0.01 mU AChE in a 100 µl assay volume (0.1 mU/ml). The assay can be performed in a 96-well or 384-well microtiter-plate format, and can be easily read with a fluorescence microplate reader at Ex/Em = 540/590 nm or with an absorbance microplate reader at OD = 575 nm.
Acetylcholinesterase assay protocol summary:
- add samples and standards to wells
- add assay mixture and incubate for 10-30 min
- analyze with microplate reader
Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.
If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure green fluorescnce (Ex/Em = 490/520 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872).
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests AbRed Indicator 1 vial Acetylcholine 1 vial Acetylcholinesterase Probe 2 units Acetylcholinesterase Standard 1 vial Assay Buffer 1 x 10ml Dilution Buffer 1 x 10ml DMSO 1 x 100µl
FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificityIsoform H is highly expressed in erythrocytes.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
- Information by UniProt
AChE measured in cell lysates showing activity per 1 million cells
AChE measured in various species showing activity per mL sample tested
Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138873 using a fluorescence microplate reader. As low as 0.01 mU/well (0.1 mU/ml) acetylcholinesterase can be detected with 20 minutes incubation (n=3).
ab138873 has been referenced in 2 publications.
- Modafferi S et al. Gene-Environment Interactions in Developmental Neurotoxicity: a Case Study of Synergy between Chlorpyrifos and CHD8 Knockout in Human BrainSpheres. Environ Health Perspect 129:77001 (2021). PubMed: 34259569
- SevIm Ç et al. In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turk J Pharm Sci 17:506-510 (2020). PubMed: 33177931