Key features and details
- Rabbit polyclonal to ACF1 / BAZ1A
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab90321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 179 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
FunctionComponent of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
Tissue specificityHighly expressed in testis and at low or undetectable levels in other tissues analyzed.
Sequence similaritiesBelongs to the WAL family.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 1 PHD-type zinc finger.
Contains 1 WAC domain.
Cellular localizationNucleus. May target the CHRAC complex to heterochromatin.
- Information by UniProt
- Acf1 antibody
- ACF1, drosophila, homolog of antibody
- ATP dependent chromatin remodelling protein antibody
ab90321 has been referenced in 1 publication.
- Hu YW et al. Long non-coding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest N/A:N/A (2018). PubMed: 30589415