Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

Overview

  • Product name

    Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal
    See all ACF1 / BAZ1A primary antibodies
  • Description

    Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ACF1/ BAZ1A aa 1500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NRL2

  • Positive control

    • HeLa, Molt4 and Jurkat whole cell lysate (ab7899); Human colon and Human testis tissues; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15826
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 203 kDa (predicted molecular weight: 178 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Target

  • Function

    Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
    Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
  • Tissue specificity

    Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
  • Sequence similarities

    Belongs to the WAL family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 WAC domain.
  • Cellular localization

    Nucleus. May target the CHRAC complex to heterochromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acf1 antibody
    • ACF1, drosophila, homolog of antibody
    • ATP dependent chromatin remodelling protein antibody
    • ATP utilizing chromatin assembly and remodeling factor 1 antibody
    • ATP-dependent chromatin-remodeling protein antibody
    • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
    • B930060C03 antibody
    • Baz1a antibody
    • BAZ1A_HUMAN antibody
    • BC065123 antibody
    • Bromodomain adjacent to zinc finger domain 1A antibody
    • Bromodomain adjacent to zinc finger domain protein 1A antibody
    • cbp146 antibody
    • CHRAC subunit ACF1 antibody
    • DKFZp586E0518 antibody
    • FLJ14383 antibody
    • Gtl5 antibody
    • hACF1 antibody
    • hWALp1 antibody
    • WALp1 antibody
    • WCRF180 antibody
    • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
    • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: BAZ1A knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab187670 was shown to specifically react with BAZ1A in wilt-type HAP1 cells. No band was observed when BAZ1A knockout samples were used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE.  Ab187670 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Molt4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 178 kDa

  • Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/50000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 178 kDa

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.

  • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ACF1 / BAZ1A with ab187670 at 1/250 (red) and DAPI staining (blue). Secondary aantibody: goat anti rabbit IgG(Alexa Fluor® 555).

References

ab187670 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - other (HeLa)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
200000 cells
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. 信哉 太田

Verified customer

Submitted Dec 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up