Recombinant Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal
See all ACF1 / BAZ1A primary antibodies -
Description
Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Daudi, Molt4 and Jurkat whole cell lysate (ab7899). IHC-P: Human colon and Human testis tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15826 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 203 kDa (predicted molecular weight: 178 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 203 kDa (predicted molecular weight: 178 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
Target
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Function
Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions. -
Tissue specificity
Highly expressed in testis and at low or undetectable levels in other tissues analyzed. -
Sequence similarities
Belongs to the WAL family.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 1 PHD-type zinc finger.
Contains 1 WAC domain. -
Cellular localization
Nucleus. May target the CHRAC complex to heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 11177 Human
- Omim: 605680 Human
- SwissProt: Q9NRL2 Human
- Unigene: 509140 Human
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Alternative names
- Acf1 antibody
- ACF1, drosophila, homolog of antibody
- ATP dependent chromatin remodelling protein antibody
see all
Images
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All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BAZ1A knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 178 kDaLanes 1-4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control ab7291 observed at 50 kDa.
ab187670 Anti-BAZ1A antibody [EPR15826] - C-terminal was shown to specifically react with BAZ1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264925 (knockout cell lysate ab257856) was used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE. ab187670 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human colon tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.
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All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : BAZ1A knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 178 kDaLanes 1 - 4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab187670 was shown to specifically react with BAZ1A in wilt-type HAP1 cells. No band was observed when BAZ1A knockout samples were used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE. Ab187670 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 178 kDa -
Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/50000 dilution + Jurkat cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 178 kDa -
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ACF1 / BAZ1A with ab187670 at 1/250 (red) and DAPI staining (blue). Secondary aantibody: goat anti rabbit IgG(Alexa Fluor® 555).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab187670 has been referenced in 1 publication.
- Chen M et al. Radiation-Induced Phosphorylation of a Prion-Like Domain Regulates Transformation by FUS-CHOP. Cancer Res 81:4939-4948 (2021). PubMed: 34385184