Overview

  • Product name
    Anti-Acid Phosphatase antibody [EPR9839]
    See all Acid Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [EPR9839] to Acid Phosphatase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Acid Phosphatase (UniProt ID: P24666).

  • Positive control
    • Human placenta, HeLa and HepG2 lysates; permeabilized Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
    • Tissue specificity
      T-lymphocytes express only isoform 2.
    • Sequence similarities
      Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid phosphatase 1 soluble antibody
      • Acid phosphatase of erythrocyte antibody
      • ACP1 antibody
      • Adipocyte acid phosphatase antibody
      • Cytoplasmic phosphotyrosyl protein phosphatase antibody
      • HAAP antibody
      • LMW-PTP antibody
      • LMW-PTPase antibody
      • Low molecular weight cytosolic acid phosphatase antibody
      • Low molecular weight phosphotyrosine protein phosphatase antibody
      • PAP1 antibody
      • PAP2 antibody
      • phosphatase, acid, of erythrocyte antibody
      • PPAC_HUMAN antibody
      • Protein tyrosine phosphatase antibody
      • PTPase antibody
      • Purple acid phosphatase antibody
      • Red cell acid phosphatase 1 antibody
      • testicular secretory protein Li 37 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).
    • All lanes : Anti-Acid Phosphatase antibody [EPR9839] (ab166896) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 18 kDa

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Locard-Paulet M  et al. Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration. Sci Signal 9:ra15 (2016). Human . Read more (PubMed: 26861043) »
    • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . Read more (PubMed: 26134519) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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