Product nameAnti-Acid Phosphatase antibody [EPR9839]
See all Acid Phosphatase primary antibodies
DescriptionRabbit monoclonal [EPR9839] to Acid Phosphatase
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Acid Phosphatase (UniProt ID: P24666).
- Human placenta, HeLa and HepG2 lysates; permeabilized Hela cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
Tissue specificityT-lymphocytes express only isoform 2.
Sequence similaritiesBelongs to the low molecular weight phosphotyrosine protein phosphatase family.
- Information by UniProt
- Acid phosphatase 1 soluble antibody
- Acid phosphatase of erythrocyte antibody
- ACP1 antibody
Flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).
All lanes : Anti-Acid Phosphatase antibody [EPR9839] (ab166896) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 18 kDa
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
This product has been referenced in:
- Locard-Paulet M et al. Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration. Sci Signal 9:ra15 (2016). Human . Read more (PubMed: 26861043) »
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . Read more (PubMed: 26134519) »