Overview

  • Product name

    Acid Phosphatase Assay Kit (Colorimetric)
    See all Acid phosphatase kits
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Enzyme activity
  • Sensitivity

    > 0.001 mM
  • Range

    0.001 mM - 1 mM
  • Assay time

    1h 10m
  • Product overview

    Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.


    The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.


    Acid phosphatase assay protocol summary:
    - add samples and standards to wells
    - add pNPP to samples
    - add acid phosphatase enzyme to standards
    - incubate for 60 min
    - add stop solution
    - analyze with microplate reader

  • Notes

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

    pNPP Assays

    pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 500 tests
    AP Assay buffer NM 1 x 100ml
    AP Enzyme (Lyophilised) Green 1 vial
    pNPP Substrate (10 tablets) Red 1 vial
    Stop Solution WM 1 x 10ml
  • Research areas

  • Relevance

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
  • Cellular localization

    ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
  • Alternative names

    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

Images

  • AP Enzyme samples 40 minute assay
  • p-Nitrophenol (pNP) standard curve

Protocols

References

This product has been referenced in:

  • Gesmundo I  et al. Proton pump inhibitors promote the growth of androgen-sensitive prostate cancer cells through ErbB2, ERK1/2, PI3K/Akt, GSK-3ß signaling and inhibition of cellular prostatic acid phosphatase. Cancer Lett 449:252-262 (2019). Read more (PubMed: 30790678) »
  • Samyn DR  et al. Key Residues and Phosphate Release Routes in the Saccharomyces cerevisiae Pho84 Transceptor: THE ROLE OF TYR179 IN FUNCTIONAL REGULATION. J Biol Chem 291:26388-26398 (2016). Read more (PubMed: 27875295) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer



If the TRAP is able to perform the same functions (phosphorylation of the substrates) as any other AP, then yes, this kit could be used. However, we cannot guarantee that it will definitely work as we have never tried it to quantify TRAP.

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Answer

Thank you for your enquiry.

Unfortunately, most of the information you asked for is proprietary info. However, I can confirm that the assay buffer does not contain protease inhibitors and its pH is 5.0.

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