• Product name
    Anti-Acid phosphatase/ACP1 antibody [EPR9839]
    See all Acid phosphatase/ACP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1
  • Host species
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Acid phosphatase/ACP1.
    Database link: P24666

  • Positive control
    • Human placenta, HeLa and HepG2 lysates; permeabilized Hela cells.
  • General notes

     This product was previously labelled as Acid Phosphatase


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab166896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
    • Tissue specificity
      T-lymphocytes express only isoform 2.
    • Sequence similarities
      Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Acid phosphatase 1 soluble antibody
      • Acid phosphatase of erythrocyte antibody
      • ACP1 antibody
      • Adipocyte acid phosphatase antibody
      • Cytoplasmic phosphotyrosyl protein phosphatase antibody
      • HAAP antibody
      • LMW-PTP antibody
      • LMW-PTPase antibody
      • Low molecular weight cytosolic acid phosphatase antibody
      • Low molecular weight phosphotyrosine protein phosphatase antibody
      • PAP1 antibody
      • PAP2 antibody
      • phosphatase, acid, of erythrocyte antibody
      • PPAC_HUMAN antibody
      • Protein tyrosine phosphatase antibody
      • PTPase antibody
      • Purple acid phosphatase antibody
      • Red cell acid phosphatase 1 antibody
      • testicular secretory protein Li 37 antibody
      see all


    • All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293 whole cell lysate
      Lane 2 : ACP1 knockout HEK-293 whole cell lysate
      Lane 3 : K562 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

      Lanes 1 - 4: Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab166896 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 18 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase/ACP1 using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.


    This product has been referenced in:
    • Locard-Paulet M  et al. Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration. Sci Signal 9:ra15 (2016). Human . Read more (PubMed: 26861043) »
    • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . Read more (PubMed: 26134519) »
    See all 2 Publications for this product

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