Overview

  • Product name

    Anti-Acidic Cytokeratin antibody [AE-1]
  • Description

    Mouse monoclonal [AE-1] to Acidic Cytokeratin
  • Host species

    Mouse
  • Specificity

    ab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Monkey, Turtle
  • Immunogen

    Recombinant full length protein corresponding to Human Acidic Cytokeratin. P13645 (CK10); P02533 (CK14); P19012 (CK15); P08789 (CK16); P08727 (CK19)
    Database link: P13645

  • Positive control

    • Skin, Squamous cell carcinoma (SCC)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    AE-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.
Flow Cyt Use 0.5-1µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Anti-Acidic Cytokeratin antibody [AE-1] (ab187381) at 1 µg/ml + A431 lysate

    Predicted band size: 66 kDa

  • Immunohistochemistry formalin-fixed, paraffin-embedded human skin tissue labeling Cytokeratin with ab187381 at 1µg/ml.

References

ab187381 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187381.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up