Anti-Acinus antibody (ab7352)
Key features and details
- Rabbit polyclonal to Acinus
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Acinus antibody
See all Acinus primary antibodies -
Description
Rabbit polyclonal to Acinus -
Host species
Rabbit -
Specificity
The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide:
TRTALHGVKWPQSNPK
, corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17. -
Positive control
- K562 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG was prepared from whole serum by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367). |
Target
-
Function
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 SAP domain. -
Post-translational
modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
-
Alternative names
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml
-
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF
-
Western blot - Anti-Acinus antibody (ab7352)Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab7352 has not yet been referenced specifically in any publications.
