Anti-Acinus antibody (ab7352)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Acinus antibody
See all Acinus primary antibodies -
DescriptionRabbit polyclonal to Acinus
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Host speciesRabbit
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SpecificityThe antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17.
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
TRTALHGVKWPQSNPK
, corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17. -
Positive control
- K562 whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified IgG was prepared from whole serum by affinity chromatography.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
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Tissue specificityUbiquitous.
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Sequence similaritiesContains 1 SAP domain.
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Post-translational
modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
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Alternative names
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF
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Western blot - Anti-Acinus antibody (ab7352)Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.
Protocols
Datasheets and documents
References
ab7352 has not yet been referenced specifically in any publications.
