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    acinus-antibody-ab7352.pdf

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Cell Biology Apoptosis Nucleus Chromatin Condensation
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Anti-Acinus antibody (ab7352)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
  • Western blot - Anti-Acinus antibody (ab7352)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)

Key features and details

  • Rabbit polyclonal to Acinus
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Acinus antibody
    See all Acinus primary antibodies
  • Description

    Rabbit polyclonal to Acinus
  • Host species

    Rabbit
  • Specificity

    The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    TRTALHGVKWPQSNPK

    , corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • K562 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified IgG was prepared from whole serum by affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Chromatin Condensation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Chromatin condensation
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Chromatin condensation
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Acinus peptide (ab8367)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).
Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).

Target

  • Function

    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 SAP domain.
  • Post-translational
    modifications

    Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9UKV3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22985 Human
    • Omim: 604562 Human
    • SwissProt: Q9UKV3 Human
    • Unigene: 124490 Human
    • Alternative names

      • ACIN 1 antibody
      • ACIN1 antibody
      • ACINU_HUMAN antibody
      • Acinus antibody
      • ACN antibody
      • Apoptotic chromatin condensation inducer 1 antibody
      • Apoptotic chromatin condensation inducer in the nucleus antibody
      • DKFZp667N107 antibody
      • fSAP152 antibody
      • functional spliceosome-associated protein 152 antibody
      • KIAA0670 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
      Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
      Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml
    • Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
      Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)

      ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF

    • Western blot - Anti-Acinus antibody (ab7352)
      Western blot - Anti-Acinus antibody (ab7352)
      Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate

      Predicted band size: 151 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
      Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7352)
      Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab7352? Please let us know so that we can cite the reference in this datasheet.

    ab7352 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Acinus antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    50 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5 %
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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