Overview

  • Product name

  • Description

    Rabbit polyclonal to Acinus
  • Host species

    Rabbit
  • Specificity

    The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    TRTALHGVKWPQSNPK

    , corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17.

  • Positive control

    • K562 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab7352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 SAP domain.
  • Post-translational
    modifications

    Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACIN 1 antibody
    • ACIN1 antibody
    • ACINU_HUMAN antibody
    • Acinus antibody
    • ACN antibody
    • Apoptotic chromatin condensation inducer 1 antibody
    • Apoptotic chromatin condensation inducer in the nucleus antibody
    • DKFZp667N107 antibody
    • fSAP152 antibody
    • functional spliceosome-associated protein 152 antibody
    • KIAA0670 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml
  • ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF

  • Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate

    Predicted band size: 151 kDa

  • Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.

References

ab7352 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5 %

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Verified customer

Submitted Mar 05 2007

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