Key features and details
- Rabbit polyclonal to Acinus
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Acinus antibody
See all Acinus primary antibodies
DescriptionRabbit polyclonal to Acinus
SpecificityThe antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'.
Tested Applications & Species
Application Species ICC/IFHuman WBHuman
- K562 whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified IgG was prepared from whole serum by affinity chromatography.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
Sequence similaritiesContains 1 SAP domain.
modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.
- Information by UniProt
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7354 at 20 µg/ml
Immunofluorescence of Acinus in K562 cells with ab7354 at 20 ug/mL.
Anti-Acinus antibody (ab7354) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa
ab7354 at 5µg/ml staining Acinus in K562 cells by ICC/IF
ab7354 has not yet been referenced specifically in any publications.