Anti-Acinus antibody (ab7354)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Acinus antibody
See all Acinus primary antibodies -
Description
Rabbit polyclonal to Acinus -
Host species
Rabbit -
Specificity
The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
DTSENRPENDVPEPP
, corresponding to amino acids 775-789 of Human Acinus L, 48 to 62 of human Acinus S’, or 17 to 31 of human Acinus S, which differ from those of mouse Acinus by one amino acid. -
Positive control
- K562 whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified IgG was prepared from whole serum by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB |
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 SAP domain. -
Post-translational
modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
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Alternative names
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7354 at 20 µg/ml -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)Immunofluorescence of Acinus in K562 cells with ab7354 at 20 ug/mL.
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Western blot - Anti-Acinus antibody (ab7354)Anti-Acinus antibody (ab7354) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)ab7354 at 5µg/ml staining Acinus in K562 cells by ICC/IF
Datasheets and documents
References
ab7354 has not yet been referenced specifically in any publications.
