The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
Contains 1 SAP domain.
Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.