- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Acinus antibody
See all Acinus primary antibodies -
DescriptionRabbit polyclonal to Acinus
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Host speciesRabbit
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SpecificityThe antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
DTSENRPENDVPEPP
, corresponding to amino acids 775-789 of Human Acinus L, 48 to 62 of human Acinus S’, or 17 to 31 of human Acinus S, which differ from those of mouse Acinus by one amino acid. -
Positive control
- K562 whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPBS with 0.02% sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified IgG was prepared from whole serum by affinity chromatography.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab7354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB |
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa). Can be blocked with Acinus (internal) peptide (human)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
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Tissue specificityUbiquitous.
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Sequence similaritiesContains 1 SAP domain.
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Post-translational
modificationsUndergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
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Alternative names
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7354 at 20 µg/ml -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)Immunofluorescence of Acinus in K562 cells with ab7354 at 20 ug/mL.
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Western blot - Anti-Acinus antibody (ab7354)Anti-Acinus antibody (ab7354) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Acinus antibody (ab7354)ab7354 at 5µg/ml staining Acinus in K562 cells by ICC/IF
References
ab7354 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"