Key features and details
- Rabbit polyclonal to Aconitase 1/ACO1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Aconitase 1/ACO1 antibody
See all Aconitase 1/ACO1 primary antibodies
DescriptionRabbit polyclonal to Aconitase 1/ACO1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
Recombinant fragment (His-tag) corresponding to Human Aconitase 1/ACO1 aa 26-243. (Expressed in E.coli)
KLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIE VPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVID HSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSG IIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIE AEAVMLGQPISMVLPQVI
Database link: P21399
- WB: Recombinant human Aconitase 1/ACO1 protein; HeLa and HepG2 cell lysates.
This product was previously labelled as Aconitase 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231353 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab231353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionIron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
- Information by UniProt
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lung tissue staining Aconitase 1/ACO1 using ab231353 10 µg/mL
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney tissue staining Aconitase 1/ACO1 using ab231353 10 µg/mL
Anti-Aconitase 1/ACO1 antibody (ab231353) at 3 µg/ml + Recombinant human Aconitase 1/ACO1 protein.
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 98 kDa
All lanes : Anti-Aconitase 1/ACO1 antibody (ab231353) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 98 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231353 has not yet been referenced specifically in any publications.